Castoldi Giovanna, Mauri Mario, D'Aliberti Deborah, Spinelli Silvia, Testa Leonardo, Gaverina Federico, Rubinacci Alessandro, Villa Isabella, Bellelli Giuseppe, Zerbini Gianpaolo, Piazza Rocco, Zatti Giovanni
Dipartimento di Medicina e Chirurgia, Università degli Studi di Milano-Bicocca, 20900 Monza, Italy.
Clinica Ortopedica, Fondazione IRCCS San Gerardo dei Tintori, 20900 Monza, Italy.
Int J Mol Sci. 2025 Apr 11;26(8):3598. doi: 10.3390/ijms26083598.
Trabecular titanium implants are widely used in orthopedic surgery and are known to promote osseointegration. In this study, we investigated whether primary human osteoblast-like cells grown inside a 3D trabecular titanium scaffold undergo changes in migration capacity, transcriptomic profile, and cellular phenotype as compared to the same osteoblasts not grown inside the scaffold. Scratch tests have shown that primary human osteoblast-like cells grown inside the 3D trabecular titanium scaffold promote the migration of cells from the external environment into the scaffold. Next generation sequencing analysis demonstrated that primary human osteoblast-like cells grown inside the 3D trabecular titanium scaffold modified the expression of genes involved in cell cycle and extracellular matrix remodeling, while maintaining a normal expression of the specific osteoblast markers, such as osteocalcin and osterix, as well as a comparable mineralization capacity. These data demonstrate that primary human osteoblast-like cells grown inside the titanium scaffold in a 3D environment acquire specific features favoring osseointegration.
松质骨钛植入物在骨科手术中广泛应用，且已知其能促进骨整合。在本研究中，我们调查了与未在三维松质骨钛支架内生长的相同成骨细胞相比，在该三维松质骨钛支架内生长的原代人成骨样细胞在迁移能力、转录组图谱和细胞表型方面是否发生变化。划痕试验表明，在三维松质骨钛支架内生长的原代人成骨样细胞可促进细胞从外部环境迁移至支架内。下一代测序分析表明，在三维松质骨钛支架内生长的原代人成骨样细胞改变了参与细胞周期和细胞外基质重塑的基因表达，同时维持了成骨细胞特异性标志物如骨钙素和osterix的正常表达以及相当的矿化能力。这些数据表明，在三维环境中钛支架内生长的原代人成骨样细胞获得了有利于骨整合的特定特征。
