Institute of Biomedical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, Taiwan.
Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan; Center of Atomic Initiative for New Materials (AI-MAT), National Taiwan University, Taipei, Taiwan.
Biosens Bioelectron. 2022 Mar 15;200:113920. doi: 10.1016/j.bios.2021.113920. Epub 2021 Dec 28.
Large-area surface-enhanced Raman spectroscopy (SERS) sensing platforms displaying ultrahigh sensitivity and signal uniformity have potentially enormous sensing applicability, but they are still challenging to prepare in a scalable manner. In this study, silver nanopaste (AgNPA) was employed to prepare a wafer-scale, ultrasensitive SERS substrate. The self-generated, high-density Ag nanocracks (NCKs) with small gaps could be created on Si wafers via a spin-coating process, and provided extremely abundant hotspots for SERS analyses with ultrahigh sensitivity-down to the level of single molecules (enhancement factor: ca. 10; detection limit: ca. 10 M)-and great signal reproducibility (variation: ca. 3.6%). Moreover, the Ag NCK arrays demonstrated broad applicability and practicability for on-site detection when combined with a portable 785 Raman spectrometer. This method allowed the highly sensitive detection of a diverse range of analytes (benzo[a]pyrene, di-2-ethylhexyl phthalate, aflatoxins B1, zearalenone, ractopamine, salbutamol, sildenafil, thiram, dimethoate, and methamidophos). In particular, pesticides are used extensively in agricultural production. Unfortunately, they can affect the environment and human health as a result of acute toxicity. Therefore, the simultaneous label-free detection of three different pesticides was demonstrated. Finally, the SERS substrates are fabricated through a simple, efficient, and scalable process that offers new opportunities for mass production.
大面积表面增强拉曼光谱（SERS）传感平台具有超高灵敏度和信号均匀性，具有巨大的传感应用潜力，但仍难以以可扩展的方式制备。在这项研究中，使用银纳米糊（AgNPA）来制备晶圆级超灵敏 SERS 基底。通过旋涂工艺，可以在 Si 晶圆上自生成具有小间隙的高密度 Ag 纳米裂纹（NCK），为 SERS 分析提供极其丰富的热点，具有超高灵敏度（低至单分子水平（增强因子：约 10；检测限：约 10^-10 M）和出色的信号重现性（变化：约 3.6%）。此外，当与便携式 785nm 拉曼光谱仪结合使用时，Ag NCK 阵列表现出广泛的适用性和现场检测的实用性。该方法允许对各种分析物（苯并[a]芘、邻苯二甲酸二（2-乙基己基）酯、黄曲霉毒素 B1、玉米赤霉烯酮、莱克多巴胺、沙丁胺醇、西地那非、福美双、乐果和甲胺磷）进行高灵敏度检测。特别是，农药在农业生产中广泛使用。不幸的是，由于急性毒性，它们会影响环境和人类健康。因此，展示了对三种不同农药的同时无标记检测。最后，SERS 基底通过简单、高效和可扩展的工艺制造，为大规模生产提供了新的机会。
