School of Chemistry, Sun Yat-Sen University, Guangzhou, 510006, China.
Mikrochim Acta. 2024 Nov 25;191(12):758. doi: 10.1007/s00604-024-06831-1.
Gold-grafted melamine sponge (AuSp) was fabricated and applied as surface-enhanced Raman spectroscopy (SERS) substrate for enzyme-linked immunoassay of 2 typical mycotoxins, zearalenone (ZEN) and T-2 toxin. The gold nanoparticles were in situ grown on the sponge, and the product showed good effect in enhancing Raman signal and good stability. COMSOL and Gaussian simulations were used to analyze the enhancing effect. Some of the experimental conditions that may affect the determination results were studied in order to meet the maximum response to mycotoxins. At optimized conditions, the limits of detection of the method were 1 μg·kg and 0.05 μg·kg for ZEN and T-2 toxin, with linear ranges of 5-100 μg·kg and 0.1-20 μg·kg, respectively, and relative standard deviations lower than 7.0%. The established method was used to determine ZEN and T-2 toxin in six kinds of cereal samples, the recoveries were 85-120%, and relative errors were less than 13.0%. These results indicated the accuracy of the established method. After 1-year storage, signal strength of AuSp for mycotoxins determination decreased by less than 10%, showing stability over a long time. Our work provided a new method for SERS determination of mycotoxins, which reduces the complexity of substrate preparation and the sample consumption compared with traditional methods, and thus has potential for on-line SERS determination.
金修饰的三聚氰胺海绵（AuSp）被制备并用作表面增强拉曼光谱（SERS）基底，用于酶联免疫吸附测定两种典型的真菌毒素，即玉米赤霉烯酮（ZEN）和 T-2 毒素。金纳米粒子原位生长在海绵上，该产物在增强拉曼信号和良好的稳定性方面表现出良好的效果。COMSOL 和高斯模拟用于分析增强效果。为了达到对真菌毒素的最大响应，研究了可能影响测定结果的一些实验条件。在优化条件下，该方法对 ZEN 和 T-2 毒素的检测限分别为 1μg·kg 和 0.05μg·kg，线性范围分别为 5-100μg·kg 和 0.1-20μg·kg，相对标准偏差低于 7.0%。建立的方法用于测定六种谷物样品中的 ZEN 和 T-2 毒素，回收率为 85-120%，相对误差小于 13.0%。这些结果表明了所建立方法的准确性。经过 1 年的储存，AuSp 对真菌毒素测定的信号强度降低了不到 10%，显示出长时间的稳定性。我们的工作为 SERS 测定真菌毒素提供了一种新方法，与传统方法相比，该方法减少了基底制备的复杂性和样品消耗，因此具有在线 SERS 测定的潜力。
