Institute of Biomedical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, 30010, Taiwan.
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, 30010, Taiwan.
Small. 2023 Jul;19(28):e2207404. doi: 10.1002/smll.202207404. Epub 2023 Mar 28.
Surface-enhanced Raman spectroscopy (SERS)-based biosensors have attracted much attention for their label-free detection, ultrahigh sensitivity, and unique molecular fingerprinting. In this study, a wafer-scale, ultrasensitive, highly uniform, paper-based, portable SERS detection platform featuring abundant and dense gold nanopearls with narrow gap distances, are prepared and deposited directly onto ultralow-surface-energy fluorosilane-modified cellulose fibers through simple thermal evaporation by delicately manipulating the atom diffusion behavior. The as-designed paper-based SERS substrate exhibits an extremely high Raman enhancement factor (3.9 × 10 ), detectability at sub-femtomolar concentrations (single-molecule level) and great signal reproductivity (relative standard deviation: 3.97%), even when operated with a portable 785-nm Raman spectrometer. This system is used for fingerprinting identification of 12 diverse analytes, including clinical medicines (cefazolin, chloramphenicol, levetiracetam, nicotine), pesticides (thiram, paraquat, carbaryl, chlorpyrifos), environmental carcinogens (benzo[a]pyrene, benzo[g,h,i]perylene), and illegal drugs (methamphetamine, mephedrone). The lowest detection concentrations reach the sub-ppb level, highlighted by a low of 16.2 ppq for nicotine. This system appears suitable for clinical applications in, for example, i) therapeutic drug monitoring for individualized medication adjustment and ii) ultra-early diagnosis for pesticide intoxication. Accordingly, such scalable, portable and ultrasensitive fibrous SERS substrates open up new opportunities for practical on-site detection in biofluid analysis, point-of-care diagnostics and precision medicine.
基于表面增强拉曼光谱（SERS）的生物传感器因其无标记检测、超高灵敏度和独特的分子指纹识别而受到广泛关注。在这项研究中，通过巧妙地控制原子扩散行为，我们制备并直接沉积了一种晶圆级的、超灵敏的、高度均匀的、基于纸张的、便携式的 SERS 检测平台，该平台具有丰富且密集的金纳米珠，其具有狭窄的间隙距离，并且通过简单的热蒸发直接沉积在超低表面能氟硅烷修饰的纤维素纤维上。所设计的基于纸张的 SERS 基底具有极高的拉曼增强因子（3.9×10 ），可在亚皮摩尔浓度（单分子水平）下检测，并具有出色的信号重现性（相对标准偏差：3.97%），即使使用便携式 785nm 拉曼光谱仪也能实现。该系统用于对 12 种不同分析物的指纹识别鉴定，包括临床药物（头孢唑林、氯霉素、左乙拉西坦、尼古丁）、农药（福美双、百草枯、西玛津、毒死蜱）、环境致癌物（苯并[a]芘、苯并[g,h,i]苝）和非法药物（甲基苯丙胺、苯丙胺）。最低检测浓度达到亚 ppb 级，尼古丁的最低检测浓度低至 16.2 ppq。该系统似乎适用于临床应用，例如：i）治疗药物监测以进行个体化药物调整和 ii）农药中毒的超早期诊断。因此，这种可扩展的、便携式的和超灵敏的纤维状 SERS 基底为生物流体分析、即时诊断和精准医学中的实际现场检测开辟了新的机会。
