Salahuddin M, Hiramatsu K, Kita K
Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
Domest Anim Endocrinol. 2022 Apr;79:106693. doi: 10.1016/j.domaniend.2021.106693. Epub 2021 Nov 19.
Glucagon-like peptide (GLP)-1 colocalizes with neurotensin (NT) in the same enteroendocrine cells (EECs) of the chicken ileum. The present study was designed to clarify the influence of dietary carbohydrate (CHO) on the colocalization pattern of GLP-1 with NT in the chicken distal ileum. Male White Leghorn chickens at 6 weeks of age (n = 15) were divided into three groups, a control and two experimental (low-CHO and CHO-free), with five chickens in each, and fed control or experimental diets for 7 d. Distal ileum was collected from each bird as a tissue sample and subjected to double immunofluorescence staining to detect GLP-1 and NT. Three types of EEC, GLP-1+/NT+, GLP-1+/NT- and GLP-1-/NT+, were demonstrated in the chicken ileum. GLP-1+/NT+ cells in the control group had a spindle-like shape with a long cytoplasmic process, but those in the experimental groups were round and lacked a cytoplasmic process. The ratio of GLP-1+/NT+ cells was significantly decreased in the two experimental groups compared with that in the control group. The ratio of GLP-1+/NT+ cells was significantly lower than those of GLP-1+/NT- and GLP-1-/NT+ cells in the two experimental groups. Most cells that were immunoreactive for GLP-1 and NT antisera lacked signals of proglucagon (PG) and NT precursor (NTP) mRNA in the experimental groups. The number of EECs expressing PG and NTP mRNA signals showed tendencies for decreases with a reduction of dietary CHO level. These findings suggest that dietary CHO could be a significant regulator of the pattern of colocalization pattern of GLP-1 with NT in the chicken ileum.
胰高血糖素样肽（GLP）-1与神经降压素（NT）在鸡回肠的同一肠内分泌细胞（EECs）中共定位。本研究旨在阐明日粮碳水化合物（CHO）对鸡远端回肠中GLP-1与NT共定位模式的影响。将6周龄的雄性白来航鸡（n = 15）分为三组，一组为对照组，两组为实验组（低CHO组和无CHO组），每组五只鸡，分别饲喂对照或实验日粮7天。从每只鸡采集远端回肠作为组织样本，进行双重免疫荧光染色以检测GLP-1和NT。在鸡回肠中发现了三种类型的EEC，即GLP-1+/NT+、GLP-1+/NT-和GLP-1-/NT+。对照组中的GLP-1+/NT+细胞呈纺锤形，有长的细胞质突起，但实验组中的细胞呈圆形且没有细胞质突起。与对照组相比，两个实验组中GLP-1+/NT+细胞的比例显著降低。在两个实验组中，GLP-1+/NT+细胞的比例显著低于GLP-1+/NT-和GLP-1-/NT+细胞的比例。在实验组中，大多数对GLP-1和NT抗血清呈免疫反应的细胞缺乏胰高血糖素原（PG）和NT前体（NTP）mRNA信号。随着日粮CHO水平的降低，表达PG和NTP mRNA信号的EEC数量有减少的趋势。这些发现表明，日粮CHO可能是鸡回肠中GLP-1与NT共定位模式的重要调节因子。
