Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
J Vet Med Sci. 2022 Mar 1;84(2):265-274. doi: 10.1292/jvms.21-0572. Epub 2021 Dec 29.
Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. Six-week-old, male White Leghorn chickens were divided into three groups: control, low-CHO and CHO-free, with five chickens in each group. Paraffin sections were made from the proximal and distal ileum of each animal and subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and GLP-2-immunoreactive cells was observed in the two experimental groups compared with that in the control. A reduction of cells expressing PG mRNA was observed in the proximal and distal ileum of the CHO-free group compared with that in the control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity was significantly lower in the distal ileum of the CHO-free group than that in the control group. These data suggest that dietary CHO is an effective stimulator for modifying L cell density in the chicken ileum.
胰高血糖素样肽（GLP）由肠道 L 细胞分泌，并刺激胃肠道中的各种生理功能。GLP 的分泌受大量营养素摄入的影响。本研究旨在阐明膳食碳水化合物（CHO）对鸡回肠 L 细胞的影响。将 6 周龄雄性白来航鸡分为三组：对照组、低 CHO 组和无 CHO 组，每组 5 只鸡。从每组动物的回肠近端和远端制作石蜡切片，并进行 GLP-1 和 GLP-2 肽的免疫组织化学和 proglucagon（PG）mRNA 的原位杂交。与对照组相比，在两个实验组中观察到 GLP-1 和 GLP-2 免疫反应性细胞明显减少。在无 CHO 组的回肠近端和远端观察到表达 PG mRNA 的细胞减少。无 CHO 组回肠远端显示 Ki-67 免疫反应性的 GLP-1 免疫反应性细胞的比例明显低于对照组。这些数据表明，膳食 CHO 是调节鸡回肠 L 细胞密度的有效刺激物。
