Department of Chemical Biology, Stratingh Institute for Chemistry, University of Groningen, Groningen, the Netherlands.
Department of Bioproduct Engineering, Engineering and Technology Institute Groningen, University of Groningen, Groningen, the Netherlands.
Carbohydr Polym. 2022 Feb 15;278:118913. doi: 10.1016/j.carbpol.2021.118913. Epub 2021 Nov 18.
Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant. The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation. Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM). This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals. In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.
双歧杆菌具有许多健康益处，例如纤维消化、抑制病原体和免疫系统成熟，特别是在新生婴儿中。双歧杆菌胞外多糖 (EPS) 通常与重要的健康效果相关，但由于缺乏对 EPS 定位的了解，其深入研究受到阻碍，而 EPS 定位对于 EPS 的有效分离很重要。在这里，我们提出了一种简单的分离程序，以获得四种商业双歧杆菌菌株（B. adolescentis、B. bifidum、B. breve 和 B. infantis）的 EPS，这些 EPS 定位于细胞膜上（通过 cryo-EM 证实）。该程序可应用于其他双歧杆菌菌株，以方便进行生物实验的分离和纯化，并为未来在营养保健品中的应用提供便利。此外，我们还证明了这四种双歧杆菌菌株的 EPS 在单糖组成和大小方面存在结构差异，突出了分离的 EPS 确定双歧杆菌特定结构-活性作用的潜力。
