Hu Yan-Hong, Ge Meng-Die, Sun Dan-Ni, Chen Lei, Cheung Peter C K, You Cui-Ping, Chen Xian-Zhong, Sun Ming-Xue, Ng Huisuan
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Int J Biol Macromol. 2022 Mar 15;201:93-103. doi: 10.1016/j.ijbiomac.2021.12.149. Epub 2021 Dec 30.
Hyperbranched polysaccharides (HBPSs) are the main components in cell wall and exopolysaccharide (EPS) of Pleurotus tuber-regium. To enhance the yield of these macromolecules, corn oil at 4% addition exhibited the best effect for production of mycelial biomass at 20.49 g/L and EPS at 0.59 g/L, which was 2.56 folds and 1.90 folds of the control, respectively. The treated hyphae were much thicker with smooth surface, while its cell wall content (43.81 ± 0.02%) was 1.96 times of the control (22.34 ± 0.01%). Moreover, a large number of lipid droplets could be visualized under the view of confocal laser scanning microscopy (CLSM). RNA-seq analysis revealed that corn oil could enter the cells and result in the up-regulation of genes on cell morphology and membrane permeability, as well as the down-regulation on expression level of polysaccharide hydrolase and genes involved in the MAPK pathway, all of which probably contribute to the increase of polysaccharides production.
超支化多糖（HBPSs）是虎奶菇细胞壁和胞外多糖（EPS）的主要成分。为提高这些大分子的产量，添加4%的玉米油对菌丝体生物量的生产效果最佳，产量为20.49 g/L，EPS产量为0.59 g/L，分别是对照的2.56倍和1.90倍。处理后的菌丝更粗壮，表面光滑，其细胞壁含量（43.81±0.02%）是对照（22.34±0.01%）的1.96倍。此外，在共聚焦激光扫描显微镜（CLSM）下可以看到大量脂滴。RNA测序分析表明，玉米油可以进入细胞，导致细胞形态和膜通透性相关基因上调，以及多糖水解酶和参与丝裂原活化蛋白激酶（MAPK）途径的基因表达水平下调，所有这些可能都有助于多糖产量的增加。
