Pan Ting, Guan Jing, Li Yujie, Sun Baolin
Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Department of Gastroenterology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Microbiol. 2021 Dec 16;12:788500. doi: 10.3389/fmicb.2021.788500. eCollection 2021.
The community-associated methicillin-resistant (CA-MRSA) causes severe pandemics primarily consisting of skin and soft tissue infections. However, the underlying pathomechanisms of the bacterium are yet to fully understood. The present study identifies LcpB protein, which belongs to the LytR-A-Psr (LCP) family, is crucial for cell wall synthesis and virulence in . The findings revealed that LcpB is a pyrophosphatase responsible for wall teichoic acid synthesis. The results also showed that LcpB regulates enzyme activity through specific key arginine sites in its LCP domain. Furthermore, knockout of in the CA-MRSA isolate ST59 resulted in enhanced hemolytic activity, enlarged of abscesses, and increased leukocyte infiltration. Meanwhile, we also found that LcpB regulates virulence in -independent manner and the key sites for pyrophosphatase of LcpB play critical roles in regulating the virulence. In addition, the results showed that the role of LcpB was different between methicillin-resistant (MRSA) and methicillin-sensitive (MSSA). This study therefore highlights the dual role of LcpB in cell wall synthesis and regulation of virulence. These insights on the underlying molecular mechanisms can thus guide the development of novel anti-infective strategies.
社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）引发主要由皮肤和软组织感染构成的严重大流行。然而，该细菌的潜在致病机制尚未完全明确。本研究鉴定出属于LytR-A-Psr（LCP）家族的LcpB蛋白，其对于[具体菌名未明确，此处原文缺失]的细胞壁合成和毒力至关重要。研究结果表明，LcpB是一种负责壁磷壁酸合成的焦磷酸酶。结果还显示，LcpB通过其LCP结构域中的特定关键精氨酸位点调节酶活性。此外，在CA-MRSA分离株ST59中敲除[具体基因未明确，此处原文缺失]导致溶血活性增强、脓肿增大以及白细胞浸润增加。同时，我们还发现LcpB以不依赖[具体因素未明确，此处原文缺失]的方式调节毒力，且LcpB焦磷酸酶的关键位点在调节毒力中起关键作用。此外，结果表明LcpB在耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）中的作用有所不同。因此，本研究突出了LcpB在细胞壁合成和毒力调节中的双重作用。这些关于潜在分子机制的见解因而能够指导新型抗感染策略的开发。
