Taché Y, Maeda-Hagiwara M, Turkelson C M
Am J Physiol. 1987 Aug;253(2 Pt 1):G241-5. doi: 10.1152/ajpgi.1987.253.2.G241.
The present study evaluates the central nervous system action of rat corticotropin-releasing factor (CRF) on gastric emptying of a liquid meal in conscious rats using the phenol red method. Intracisternal injection of CRF (63-210 pmol) dose-dependently inhibited gastric emptying of a liquid meal by 37-80%. Peptide action was rapid in onset, long acting, and not mimicked by intracisternal injection of growth hormone-releasing factor. Intracisternal CRF-induced inhibition of gastric emptying was reversed by subdiaphragmatic vagotomy but not by naloxone pretreatment or adrenalectomy. Intravenous injection of CRF (21-630 pmol) also dose-dependently inhibited gastric emptying. CRF antiserum blocked the effect of intravenous but not of intracisternal injection of CRF (63 pmol). These results demonstrated that CRF injected in a picomole amount into the cerebrospinal fluid acts within the brain to inhibit gastric emptying of a liquid meal through vagal-dependent pathways.
本研究采用酚红法评估大鼠促肾上腺皮质激素释放因子（CRF）对清醒大鼠液体餐胃排空的中枢神经系统作用。脑池内注射CRF（63 - 210皮摩尔）可剂量依赖性地抑制液体餐的胃排空，抑制率为37% - 80%。肽类作用起效迅速、持续时间长，且脑池内注射生长激素释放因子不能模拟该作用。脑池内注射CRF诱导的胃排空抑制可通过膈下迷走神经切断术逆转，但纳洛酮预处理或肾上腺切除术不能逆转。静脉注射CRF（21 - 630皮摩尔）也可剂量依赖性地抑制胃排空。CRF抗血清可阻断静脉注射CRF（63皮摩尔）的作用，但不能阻断脑池内注射CRF的作用。这些结果表明，皮摩尔量的CRF注入脑脊液后在脑内起作用，通过迷走神经依赖性途径抑制液体餐的胃排空。
