Panjwani N, Rodrigues M, Free K, Krachmer J H, Albert D, Baum J
Arch Ophthalmol. 1987 May;105(5):688-91. doi: 10.1001/archopht.1987.01060050106047.
We analyzed lectin binding patterns of amyloid glycoconjugates in patients with lattice dystrophy of the cornea. Results of paraffin and frozen sections differed in some instances. With paraffin sections, three lectins--wheat germ agglutinin (WGA), Ricinus communis agglutinin I (RCA-I), and concanavalin A (Con A)--stained the abnormal deposits. In frozen sections, the abnormal deposits were stained by five lectins--WGA, RCA-I, Con A, peanut agglutinin (PNA), and soybean agglutinin (SBA). In paraffin sections, PNA and SBA did not stain amyloid deposits. In both paraffin and frozen sections, some lectin-positive deposits corresponded to the Congo red-positive material, whereas others were present surrounding and encroaching on the Congo red-reactive material. This study demonstrates that WGA-, RCA-I-, Con A-, PNA-, and SBA-positive abnormal deposits are present in corneas with lattice dystrophy. Since lectins bind to specific sugar residues, we conclude that the abnormal deposits consist, at least in part, of glycoconjugates and that these glycoconjugates contain oligosaccharides with N-acetylglucosamine/sialic acid, mannose/glucose and terminal beta-galactose residues and chains with terminal beta-galactose-N-acetylgalactosamine disaccharides.
我们分析了角膜格子状营养不良患者淀粉样糖缀合物的凝集素结合模式。石蜡切片和冰冻切片的结果在某些情况下有所不同。对于石蜡切片，三种凝集素——麦胚凝集素（WGA）、蓖麻凝集素I（RCA-I）和刀豆球蛋白A（Con A）——可使异常沉积物染色。在冰冻切片中，五种凝集素——WGA、RCA-I、Con A、花生凝集素（PNA）和大豆凝集素（SBA）——可使异常沉积物染色。在石蜡切片中，PNA和SBA不能使淀粉样沉积物染色。在石蜡切片和冰冻切片中，一些凝集素阳性沉积物与刚果红阳性物质相对应，而其他沉积物则存在于刚果红反应性物质周围并侵入其中。本研究表明，角膜格子状营养不良患者的角膜中存在WGA、RCA-I、Con A、PNA和SBA阳性的异常沉积物。由于凝集素与特定的糖残基结合，我们得出结论，异常沉积物至少部分由糖缀合物组成，并且这些糖缀合物含有带有N-乙酰葡糖胺/唾液酸、甘露糖/葡萄糖以及末端β-半乳糖残基的寡糖，以及带有末端β-半乳糖-N-乙酰半乳糖胺二糖的链。
