Université de Paris, Infection Antimicrobials Modelling Evolution (IAME), Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
Assistance Publique - Hôpitaux de Paris (AP-HP), University Hospital Bichat-Claude Bernard, Laboratoire de Virologie, Paris, France.
Front Cell Infect Microbiol. 2021 Dec 16;11:792202. doi: 10.3389/fcimb.2021.792202. eCollection 2021.
Since its emergence in China at the end of 2019, SARS-CoV-2 has rapidly spread across the world to become a global public health emergency. Since then, the pandemic has evolved with the large worldwide emergence of new variants, such as the Alpha (B.1.1.7 variant), Beta (B.1.351 variant), and Gamma (P.1 variant), and some other under investigation such as the A.27 in France. Many studies are focusing on antibody neutralisation changes according to the spike mutations, but to date, little is known regarding their respective replication capacities. In this work, we demonstrate that the Alpha variant provides an earlier replication , on Vero E6 and A549 cells, than Beta, Gamma, A.27, and historical lineages. This earlier replication was associated with higher infectious titres in cell-culture supernatants, in line with the higher viral loads observed among Alpha-infected patients. Interestingly, Beta and Gamma variants presented similar kinetic and viral load than the other non-Alpha-tested variants.
自 2019 年底在中国出现以来，SARS-CoV-2 迅速在全球蔓延，成为全球公共卫生紧急事件。从那时起，随着新变种在全球范围内的大量出现，如 Alpha（B.1.1.7 变种）、Beta（B.1.351 变种）和 Gamma（P.1 变种），以及其他一些正在研究中的变种，如法国的 A.27，大流行一直在演变。许多研究都集中在根据刺突突变来研究抗体中和变化上，但迄今为止，对于它们各自的复制能力知之甚少。在这项工作中，我们证明 Alpha 变种在 Vero E6 和 A549 细胞中的复制更早，比 Beta、Gamma、A.27 和历史谱系更早。这种早期复制与细胞培养上清液中更高的感染性滴度有关，与 Alpha 感染患者中观察到的更高病毒载量一致。有趣的是，Beta 和 Gamma 变种的动力学和病毒载量与其他非 Alpha 测试变种相似。
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