Li Xiaolei, Xiong Feng, Wang Shuguang, Zhang Zhuojun, Dai Jihang, Chen Hui, Wang Jingcheng, Wang Qiang, Yuan Huihua
Department of Orthopedics and Orthopedic Institute, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou, China.
School of Life Sciences, Nantong University, Nantong, China.
Front Bioeng Biotechnol. 2021 Dec 15;9:767641. doi: 10.3389/fbioe.2021.767641. eCollection 2021.
To regenerate bone tissues, we investigated the osteogenic differentiation of induced-pluripotent-stem-cell-derived mesenchymal stem cells (iPSC-MSCs) and bone regeneration capacities using N-acetyl cysteine (NAC)-loaded biomimetic nanofibers of hydroxyapatite/silk fibroin (HAp/SF). The addition of HAp and NAC decreased the diameters of the electrospun fibers and enhanced the mechanical properties of the silk scaffold. The release kinetic curve indicated that NAC was released from NAC/HAp/SF nanofibers in a biphasic pattern, with an initial burst release stage and a later sustained release stage. This pattern of release of NAC encapsulated on the NAC/HAp/SF scaffolds prolonged the release of high concentrations of NAC, thereby largely affecting the osteogenic differentiation of iPSC-MSCs and bone regeneration. Thus, a new silk electrospun scaffold was developed. HAp was used as a separate nanocarrier for recharging the NAC concentration, which demonstrated the promising potential for the use of NAC/HAp/SF for bone tissue engineering.
为了再生骨组织，我们研究了诱导多能干细胞来源的间充质干细胞（iPSC-MSCs）的成骨分化以及使用负载N-乙酰半胱氨酸（NAC）的羟基磷灰石/丝素蛋白（HAp/SF）仿生纳米纤维的骨再生能力。HAp和NAC的添加减小了电纺纤维的直径，并增强了丝素支架的力学性能。释放动力学曲线表明，NAC以双相模式从NAC/HAp/SF纳米纤维中释放，包括初始的突释阶段和随后的缓释阶段。NAC封装在NAC/HAp/SF支架上的这种释放模式延长了高浓度NAC的释放时间，从而在很大程度上影响了iPSC-MSCs的成骨分化和骨再生。因此，开发了一种新型的丝素电纺支架。HAp用作单独的纳米载体以补充NAC浓度，这证明了NAC/HAp/SF在骨组织工程中的应用潜力巨大。
