Anderson S J, Lawton A R
Clin Immunol Immunopathol. 1987 Sep;44(3):259-71. doi: 10.1016/0090-1229(87)90070-5.
Bacterial lipopolysaccharide (LPS) augments production of IgM and IgG by two- to seven-fold in cultures of peripheral blood lymphocytes (PBL) stimulated by pokeweed mitogen (PWM), but only if monocytes are rigorously depleted. When PBL were separated into adherent cell (AC), B-cell-enriched, and T-cell-enriched fractions, pulsed with LPS, and recombined in culture with PWM, increased generation of plasma cells was seen only in cultures containing LPS-treated B cells. This effect of LPS appears to be independent of soluble factors. Supernatants from LPS-stimulated B cells or AC did not consistently increase PWM responses when cultured with fresh B cells in the presence of polymyxin B. Furthermore, pulsing of B cells with purified interleukin 1 from two different commercial sources failed to augment PWM-induced differentiation. When B cells were depleted of surface IgD (sIgD)-bearing cells by panning, no effect on LPS-mediated augmentation of PWM-driven differentiation was seen. B cells were also fractionated by rosetting with mouse erythrocytes. Treatment of BMR+ cells with LPS did not induce them to respond to PWM, while treatment of BMR- cells with LPS augmented generation of plasma cells. These results indicate that LPS acts directly to augment differentiation of PWM-responsive B cells, rather than recruiting sIgD+, BMR+ cells to become PWM responsive.
细菌脂多糖（LPS）可使商陆丝裂原（PWM）刺激的外周血淋巴细胞（PBL）培养物中IgM和IgG的产生增加2至7倍，但前提是要严格去除单核细胞。当将PBL分离为贴壁细胞（AC）、富含B细胞的部分和富含T细胞的部分，用LPS脉冲处理，然后在培养中与PWM重新组合时，仅在含有经LPS处理的B细胞的培养物中观察到浆细胞生成增加。LPS的这种作用似乎与可溶性因子无关。在多粘菌素B存在的情况下，将来自LPS刺激的B细胞或AC的上清液与新鲜B细胞一起培养时，并未持续增加PWM反应。此外，用来自两种不同商业来源的纯化白细胞介素1对B细胞进行脉冲处理，未能增强PWM诱导的分化。当通过淘选去除B细胞表面带有IgD（sIgD）的细胞时，未观察到对LPS介导的PWM驱动分化增强的影响。还通过与小鼠红细胞进行花环形成对B细胞进行分级分离。用LPS处理BMR +细胞不会诱导它们对PWM作出反应，而用LPS处理BMR-细胞则会增加浆细胞的生成。这些结果表明，LPS直接作用以增强对PWM有反应的B细胞的分化，而不是招募sIgD +、BMR +细胞使其对PWM产生反应。
