使用DNA-PAINT量化聚乙二醇（PEG）结构对纳米颗粒配体可用性的影响。

Andrian Teodora, Pujals Silvia, Albertazzi Lorenzo

Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology Baldiri Reixac 15-21 08028 Barcelona Spain

Department of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology 5612AZ Eindhoven The Netherlands.

Nanoscale Adv. 2021 Nov 1;3(24):6876-6881. doi: 10.1039/d1na00696g. eCollection 2021 Dec 7.

The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate, especially at a single particle level. Here, we apply DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT), a super-resolution microscopy (SRM) technique, to study the surface functionality in poly(lactide--glycolide)-poly(ethylene glycol) (PLGA-PEG) NPs with different PEG structures. We demonstrated how the length of the PEG spacer can influence the accessibility of surface chemical functionality, highlighting the importance of SRM techniques to support the rational design of functionalized NPs.

聚乙二醇（PEG）结构对纳米颗粒（NP）功能的重要性是已知的，但仍难以研究，尤其是在单颗粒水平上。在此，我们应用纳米级形貌成像的DNA点积累技术（DNA-PAINT），一种超分辨率显微镜（SRM）技术，来研究具有不同PEG结构的聚（丙交酯-乙交酯）-聚（乙二醇）（PLGA-PEG）纳米颗粒的表面功能。我们展示了PEG间隔臂的长度如何影响表面化学功能的可及性，突出了SRM技术对支持功能化纳米颗粒合理设计的重要性。