Muench Lukas N, Kriscenski Danielle, Tamburini Lisa, Berthold Daniel P, Rupp Marco-Christopher, Mancini Michael R, Cote Mark P, McCarthy Mary Beth, Mazzocca Augustus D
Department of Orthopedic Surgery, UConn Health Center, Farmington, Connecticut, U.S.A.
Department of Orthopaedic Sports Medicine, Technical University of Munich, Germany.
Arthrosc Sports Med Rehabil. 2021 Nov 14;3(6):e1975-e1980. doi: 10.1016/j.asmr.2021.09.020. eCollection 2021 Dec.
To evaluate the effect of magnesium on cellular adhesion and proliferation of human subacromial bursal tissue (SBT), osteoblasts, and tenocytes on nonabsorbable suture tape commonly used in rotator cuff surgery.
Human SBT cells, primary human osteoblasts (HOBs), and primary human tenocytes were isolated from tissue samples and cultured in growth media. Commercially available collagen-coated nonabsorbable suture tape was cut into one-inch pieces, placed into 48-well culture dishes, sterilized under ultraviolet light, and treated with (+) or without (-) magnesium. For the (+) magnesium group, a one-time dose of 5 mM sterile magnesium chloride was added. Subsequently, cells were plated at a density of 20,000 cells/cm. For each cell source (SBT, HOBs, tenocytes) cellular proliferation and adhesion assays on suture tape treated (+) or (-) magnesium were performed.
SBT, HOBs, and tenocytes each demonstrated the ability to adhere and proliferate on suture tape. Augmenting suture tape with magnesium resulted in a significantly increased cellular adhesion of SBT compared with nonaugmented sutures ( = .001), whereas no significant differences were observed for HOBs ( = .081) and tenocytes ( = .907). Augmentation with magnesium demonstrated no significant difference in cellular proliferation of SBT ( = .856), HOBs ( = .672), and tenocytes ( = .251) compared with nonaugmented sutures.
SBT, osteoblasts, and tenocytes each demonstrated the ability to adhere and proliferate on suture tape. In addition, augmenting the suture with magnesium resulted in a significantly increased cellular adhesion of SBT compared with nonaugmented sutures, whereas no significant differences were observed for osteoblasts and tenocytes. Further, magnesium did not impair the proliferative activity of SBT, osteoblasts, and tenocytes on suture tape used in rotator cuff surgery.
Modifying the surface of the suture used for repair with application of magnesium may be an inexpensive and technically feasible option to improve the use of SBT for biologic augmentation of rotator cuff repair.
评估镁对肩峰下滑囊组织（SBT）、成骨细胞和肌腱细胞在肩袖手术中常用的不可吸收缝合带上的细胞黏附和增殖的影响。
从组织样本中分离出人SBT细胞、原代人成骨细胞（HOB）和原代人肌腱细胞，并在生长培养基中培养。将市售的胶原涂层不可吸收缝合带切成1英寸长的片段，放入48孔培养板中，在紫外线下灭菌，并用（+）或不用（-）镁处理。对于（+）镁组，加入一次性剂量的5 mM无菌氯化镁。随后，以20000个细胞/cm的密度接种细胞。针对每种细胞来源（SBT、HOB、肌腱细胞），对经（+）或（-）镁处理的缝合带进行细胞增殖和黏附试验。
SBT、HOB和肌腱细胞均表现出在缝合带上黏附和增殖的能力。与未添加镁的缝合线相比，用镁增强缝合带可使SBT的细胞黏附显著增加（P = 0.001），而HOB（P = 0.081）和肌腱细胞（P = 0.907）未观察到显著差异。与未添加镁的缝合线相比，添加镁在SBT（P = 0.856）、HOB（P = 0.672）和肌腱细胞（P = 0.251）的细胞增殖方面未显示出显著差异。
SBT、成骨细胞和肌腱细胞均表现出在缝合带上黏附和增殖的能力。此外，与未添加镁的缝合线相比，用镁增强缝合带可使SBT的细胞黏附显著增加，而成骨细胞和肌腱细胞未观察到显著差异。此外，镁不会损害SBT、成骨细胞和肌腱细胞在肩袖手术中使用的缝合带上的增殖活性。
应用镁修饰用于修复的缝合线表面可能是一种廉价且技术上可行的选择，以改善SBT在肩袖修复生物增强中的应用。
