胰岛素及胰岛素样生长因子1（IGF-1）对人肩峰下囊组织细胞增殖与迁移的影响
The Effect of Insulin and Insulin-like Growth Factor 1 (IGF-1) on Cellular Proliferation and Migration of Human Subacromial Bursa Tissue.
作者信息
Muench Lukas N, Tamburini Lisa, Kriscenski Danielle, Landry Arthur, Berthold Daniel P, Kia Cameron, Cote Mark P, McCarthy Mary Beth, Mazzocca Augustus D
机构信息
Department of Orthopaedic Surgery, UConn Health Center, Farmington Connecticut, U.S.A.
Department of Orthopaedic Sports Medicine, Technical University of Munich, Munich, Germany.
出版信息
Arthrosc Sports Med Rehabil. 2021 Mar 22;3(3):e781-e789. doi: 10.1016/j.asmr.2021.01.019. eCollection 2021 Jun.
PURPOSE
To evaluate the effect of a one-time dose of insulin or insulin-like growth factor 1 (IGF-1) on cellular proliferation and migration of subacromial bursa tissue (SBT) over time.
METHODS
SBT was harvested from over the rotator cuff tendon in 4 consecutive patients undergoing primary arthroscopic rotator cuff repair. SBT was cultured for 3 weeks in complete media until reaching confluence. The culture dishes were stored in a humidified, low oxygen tension (5% CO) incubator at 37°C. SBT of each patient underwent treatment with a one-time dose of insulin or IGF-1, whereas nontreated SBT served as a negative control. Cellular proliferation and migration were evaluated after 24, 48, 72, and 96 hours of incubation. SBT-derived cells migrated in the detection field were visualized using fluorescent microscopy.
RESULTS
Cellular proliferation at 24, 48, 72, and 96 hours was 1.40 ± 0.27, 1.00 ± 0.20, 1.47 ± 0.31, and 1.68 ± 0.28 for IGF-1; 1.44 ± 0.24, 1.15 ± 0.27, 1.60 ± 0.36, and 1.61 ± 0.32 for insulin; and 1.51 ± 0.35, 1.29 ± 0.33, 1.53 ± 0.35, and 1.57 ± 0.38 for nontreated SBT. Untreated SBT demonstrated a significantly greater proliferation when compared with IGF-1 and insulin within the first 48 hours, although this effect was found to subside by 96 hours. Cellular migration at 24, 48, 72, and 96 hours was 575.7 ± 45.0, 641.6 ± 77.7, 728.3 ± 122.9, and 752.3 ± 114.5 for IGF-1; 528.4 ± 31.3, 592.5 ± 69.8, 664.2 ± 115.2, and 695.6 ± 148.2 for insulin; and 524.4 ± 41.9, 564.4 ± 49.8, 653.2 ± 81.5, and 685.7 ± 115.5 for nontreated SBT. Insulin showed no difference in migration at each timepoint compared to nontreated SBT ( > .05, respectively).
CONCLUSIONS
Insulin and IGF-1 initially inhibit cellular proliferation of human SBT, although this effect was found to subside by 96 hours. Further, neither insulin nor IGF-1 changed the slope of cellular migration over time. However, each treatment group demonstrated a significant increase in cellular proliferation and migration.
CLINICAL RELEVANCE
In the setting of biologic augmentation of rotator cuff repair, the compatibility and synergistic effect of insulin on human SBT is highly limited.
目的
评估一次性给予胰岛素或胰岛素样生长因子1（IGF-1）对肩峰下滑囊组织（SBT）细胞增殖和迁移随时间变化的影响。
方法
从4例连续接受初次关节镜下肩袖修复术患者的肩袖肌腱上方获取SBT。将SBT在完全培养基中培养3周直至汇合。培养皿置于37°C、湿度适宜、低氧张力（5% CO）的培养箱中。每位患者的SBT接受一次性剂量的胰岛素或IGF-1治疗，未治疗的SBT作为阴性对照。在孵育24、48、72和96小时后评估细胞增殖和迁移情况。使用荧光显微镜观察检测视野中SBT来源细胞的迁移。
结果
IGF-1在24、48、72和96小时的细胞增殖率分别为1.40±0.27、1.00±0.20、1.47±0.31和1.68±0.28；胰岛素分别为1.44±0.24、1.15±0.27、1.60±0.36和1.61±0.32；未治疗的SBT分别为1.51±0.35、1.29±0.33、1.53±0.35和1.57±0.38。与IGF-1和胰岛素相比，未治疗的SBT在最初48小时内增殖明显更多，尽管这种效应在96小时时减弱。IGF-1在24、48、72和96小时的细胞迁移数分别为575.7±45.0、641.6±77.7、728.3±122.9和752.3±114.5；胰岛素分别为528.4±31.3、592.5±69.8、664.2±115.2和695.6±148.2；未治疗的SBT分别为524.4±41.9、564.4±49.8、653.2±81.5和685.7±115.5。与未治疗的SBT相比，胰岛素在每个时间点的迁移均无差异（P均>.05）。
结论
胰岛素和IGF-1最初抑制人SBT的细胞增殖，尽管这种效应在96小时时减弱。此外，胰岛素和IGF-1均未改变细胞迁移随时间的斜率。然而，每个治疗组的细胞增殖和迁移均显著增加。
临床意义
在肩袖修复的生物增强背景下，胰岛素对人SBT的兼容性和协同作用非常有限。
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