• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于光电流测量和原子力显微镜表征的反应中心的纯化和制备。

Purification and preparation of reaction centers for photocurrent measurements and atomic force microscopy characterization.

机构信息

Advanced Materials and Process Engineering Laboratory, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.

出版信息

STAR Protoc. 2021 Dec 16;3(1):101044. doi: 10.1016/j.xpro.2021.101044. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2021.101044
PMID:34977685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8689352/
Abstract

The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multilayers and non-specifically adsorbed RCs. The resulting electrode surfaces are analyzed for photocurrent generation using an intensity-modulated light and lock-in amplifier. Atomic force microscopy (AFM) is used to characterize the surface and the formation of RC structural assemblies. For complete details on the use and execution of this profile, please refer to Jun et al. (2021).

摘要

在生物混合太阳能电池中形成由光合反应中心 (RC) 组成的定义表面具有挑战性。在这里，我们从定向结合的工程 RC 的生产开始。将 RC 沉积在金电极上，并使用 6-巯基-1-己醇 (MCH) 置换多层和非特异性吸附的 RC。使用强度调制光和锁相放大器分析产生光电流的电极表面。原子力显微镜 (AFM) 用于表征表面和 RC 结构组件的形成。有关此配置文件的使用和执行的完整详细信息，请参阅 Jun 等人。(2021)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/cef76837b79e/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/c38448abf02a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/8a70bf73af4e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/35ff387bb72e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/c327121b4733/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/08c806953f32/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/7f587254e915/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/ff6027eb9c95/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/4a18bf887521/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/9b0380ca08bf/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/8f7658d07a7c/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/0db155916c42/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/0d2507a65840/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/02d3562e3e06/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/08ad4b7a7ea6/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/cef76837b79e/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/c38448abf02a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/8a70bf73af4e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/35ff387bb72e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/c327121b4733/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/08c806953f32/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/7f587254e915/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/ff6027eb9c95/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/4a18bf887521/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/9b0380ca08bf/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/8f7658d07a7c/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/0db155916c42/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/0d2507a65840/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/02d3562e3e06/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/08ad4b7a7ea6/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db9a/8689352/cef76837b79e/gr14.jpg

相似文献

1
Purification and preparation of reaction centers for photocurrent measurements and atomic force microscopy characterization.用于光电流测量和原子力显微镜表征的反应中心的纯化和制备。
STAR Protoc. 2021 Dec 16;3(1):101044. doi: 10.1016/j.xpro.2021.101044. eCollection 2022 Mar 18.
2
Photocurrent and electronic activities of oriented-His-tagged photosynthetic light-harvesting/reaction center core complexes assembled onto a gold electrode.取向 His 标签的光合光捕获/反应中心核心复合物在金电极上组装的光电流和电子活性。
Biomacromolecules. 2012 Feb 13;13(2):432-8. doi: 10.1021/bm201457s. Epub 2012 Feb 1.
3
The electronic behavior of a photosynthetic reaction center monitored by conductive atomic force microscopy.通过导电原子力显微镜监测光合反应中心的电子行为。
J Nanosci Nanotechnol. 2009 Jan;9(1):97-107. doi: 10.1166/jnn.2009.j084.
4
Cu2+ site in photosynthetic bacterial reaction centers from Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodopseudomonas viridis.来自球形红杆菌、荚膜红杆菌和绿假单胞菌的光合细菌反应中心中的铜离子位点。
Biochemistry. 2001 May 22;40(20):6132-41. doi: 10.1021/bi0029191.
5
Aberrant assembly complexes of the reaction center light-harvesting 1 PufX (RC-LH1-PufX) core complex of Rhodobacter sphaeroides imaged by atomic force microscopy.通过原子力显微镜成像的球形红细菌反应中心光捕获1 PufX（RC-LH1-PufX）核心复合物的异常组装复合体。
J Biol Chem. 2014 Oct 24;289(43):29927-36. doi: 10.1074/jbc.M114.596585. Epub 2014 Sep 5.
6
Modelling of the cathodic and anodic photocurrents from Rhodobacter sphaeroides reaction centres immobilized on titanium dioxide.固定在二氧化钛上的球形红杆菌反应中心的阴极和阳极光电流的建模。
Photosynth Res. 2018 Oct;138(1):103-114. doi: 10.1007/s11120-018-0550-8. Epub 2018 Jul 3.
7
Correlating structural assemblies of photosynthetic reaction centers on a gold electrode and the photocurrent - potential response.金电极上光合反应中心的结构组装与光电流-电势响应的相关性
iScience. 2021 May 4;24(5):102500. doi: 10.1016/j.isci.2021.102500. eCollection 2021 May 21.
8
AFM characterization of tilt and intrinsic flexibility of Rhodobacter sphaeroides light harvesting complex 2 (LH2).球形红细菌光捕获复合物2（LH2）倾斜和固有柔韧性的原子力显微镜表征
J Mol Biol. 2003 Jan 17;325(3):569-80. doi: 10.1016/s0022-2836(02)01241-x.
9
High-Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot-Bacterial Reaction Center Nanoconjugates.生物杂化量子点-细菌反应中心纳米复合物中的高效激发能量转移。
J Phys Chem Lett. 2021 Jun 17;12(23):5448-5455. doi: 10.1021/acs.jpclett.1c01407. Epub 2021 Jun 3.
10
Electron-nuclear and electron-electron double resonance spectroscopies show that the primary quinone acceptor QA in reaction centers from photosynthetic bacteria Rhodobacter sphaeroides remains in the same orientation upon light-induced reduction.电子-核和电子-电子双共振光谱表明，来自光合细菌球形红杆菌的反应中心的最初醌受体 QA 在光诱导还原时仍保持相同的取向。
J Phys Chem B. 2010 Dec 23;114(50):16894-901. doi: 10.1021/jp107051r. Epub 2010 Nov 24.

本文引用的文献

1
Correlating structural assemblies of photosynthetic reaction centers on a gold electrode and the photocurrent - potential response.金电极上光合反应中心的结构组装与光电流-电势响应的相关性
iScience. 2021 May 4;24(5):102500. doi: 10.1016/j.isci.2021.102500. eCollection 2021 May 21.
2
Introduction of the Menaquinone Biosynthetic Pathway into and Synthesis of Menaquinone for Incorporation into Heterologously Expressed Integral Membrane Proteins.导入menaquinone 生物合成途径和合成menaquinone 以整合到异源表达的整合膜蛋白中。
ACS Synth Biol. 2020 May 15;9(5):1190-1200. doi: 10.1021/acssynbio.0c00066. Epub 2020 Apr 21.
3
In vivo assembly of a truncated H subunit mutant of the Rhodobacter sphaeroides photosynthetic reaction centre and direct electron transfer from the Q quinone to an electrode.
在活体内组装截短的 Rhodobacter sphaeroides 光合反应中心的 H 亚基突变体，并使 Q 醌直接向电极传递电子。
Photosynth Res. 2018 Aug;137(2):227-239. doi: 10.1007/s11120-018-0493-0. Epub 2018 Mar 9.
4
Use of new strains of Rhodobacter sphaeroides and a modified simple culture medium to increase yield and facilitate purification of the reaction centre.利用新型球形红杆菌菌株和改良的简单培养基来提高反应中心的产量并便于其纯化。
Photosynth Res. 2014 May;120(1-2):197-205. doi: 10.1007/s11120-013-9866-6. Epub 2013 Jun 14.
5
The petite purple photosynthetic powerpack.小巧的紫色光合能量包。
Biochem Soc Trans. 2009 Apr;37(Pt 2):400-7. doi: 10.1042/BST0370400.
6
Kinetic analysis of the thermal stability of the photosynthetic reaction center from Rhodobacter sphaeroides.球形红杆菌光合反应中心热稳定性的动力学分析
Biophys J. 2006 Jun 1;90(11):4155-66. doi: 10.1529/biophysj.105.070029. Epub 2006 Mar 13.
7
Pigment content and molar extinction coefficients of photochemical reaction centers from Rhodopseudomonas spheroides.球形红假单胞菌光化学反应中心的色素含量和摩尔消光系数
Biochim Biophys Acta. 1973 Jun 28;305(3):597-609. doi: 10.1016/0005-2728(73)90079-0.