Phylactos A C, Unger W G, Grierson I
Curr Eye Res. 1987 Jul;6(7):865-83. doi: 10.3109/02713688709034856.
Intravitreal injection of 5 micrograms of Shigella endotoxin, in the rabbit eye, induced an acute inflammatory response which was characterised by conjunctival hyperaemia, limbal and ciliary vascular injection, iritis, aqueous flare, miosis and reduction in intraocular pressure. Iris-ciliary body tissues, from normal and inflamed eyes, were fractionated into subcellular enriched fractions and the activities and distribution of selected enzymes were estimated. Alkaline phosphatase, a plasma membrane-bound enzyme, showed an increase in activity, whereas succinate dehydrogenase and Mn-Superoxide dismutase, both mitochondrial-bound enzymes, exhibited decreased activities. Lysosomal acid phosphatase displayed an increase in free activity and retention of latent activity inside the organelle. No alteration in free activity was shown by acid cathepsin. The cholinesterases did not exhibit any changes in activities nor did the cytosolic enzymes Cu/Zn-superoxide dismutase and lactate dehydrogenase. The decrease activity of the respiratory mitochondrial enzyme succinate dehydrogenase may contribute to the reduction in intraocular pressure, and the ability of the lysosomal organelles to retain their hydrolytic enzymes, ensures recovery of the cell from acute inflammatory attack.
给兔眼玻璃体内注射5微克志贺氏菌内毒素可引发急性炎症反应，其特征为结膜充血、角膜缘及睫状血管充血、虹膜炎、房水闪光、瞳孔缩小和眼压降低。将正常眼和发炎眼的虹膜 - 睫状体组织分离成亚细胞富集组分，并估计所选酶的活性和分布。碱性磷酸酶是一种与质膜结合的酶，其活性增加，而琥珀酸脱氢酶和锰 - 超氧化物歧化酶这两种与线粒体结合的酶，活性均降低。溶酶体酸性磷酸酶的游离活性增加，且在细胞器内保留潜在活性。酸性组织蛋白酶的游离活性未显示出变化。胆碱酯酶的活性未表现出任何变化，胞质酶铜/锌 - 超氧化物歧化酶和乳酸脱氢酶也未发生变化。呼吸线粒体酶琥珀酸脱氢酶活性的降低可能导致眼压降低，溶酶体细胞器保留其水解酶的能力可确保细胞从急性炎症攻击中恢复。
