Higgins A J, Lees P, Sharma S C, Taylor J B
Equine Vet J. 1987 Jul;19(4):303-6. doi: 10.1111/j.2042-3306.1987.tb01416.x.
An accurate and reliable method for the separation of flunixin from, and measurement in, equine inflammatory exudate and plasma by high performance liquid chromatography has been developed. Flunixin can be detected in concentrations as low as 0.05 micrograms/ml using an ultraviolet spectrophotometric detector at 285 nm. Samples were acidified with 2M hydrochloric acid and extracted with dichloromethane. The extract was evaporated and reconstituted in acetonitrile. Iminodibenzyl was used as internal standard. The mean recovery of flunixin from plasma was 97.6 +/- 3.9 per cent. Particular advantages of the method are the short analysis time and ease of sample preparation. Data were obtained on the distribution of flunixin between plasma and acute inflammatory exudate following administration of a single intravenous dose of 1.1 mg/kg bodyweight flunixin meglumine. The drug was cleared more slowly from exudate than from plasma.
已开发出一种通过高效液相色谱法从马炎性渗出液和血浆中分离氟尼辛并进行测定的准确可靠方法。使用285nm的紫外分光光度检测器，氟尼辛的检测浓度可低至0.05微克/毫升。样品用2M盐酸酸化，并用二氯甲烷萃取。萃取液蒸发后用乙腈复溶。亚氨基二苄用作内标。氟尼辛从血浆中的平均回收率为97.6±3.9%。该方法的特别优点是分析时间短且样品制备容易。在静脉注射1.1mg/kg体重的氟尼辛葡甲胺单次剂量后，获得了氟尼辛在血浆和急性炎性渗出液之间分布的数据。该药物从渗出液中的清除比从血浆中更慢。
