Siegel J N, Malemud C J, Kammer G M
Immunol Lett. 1987 Jun;15(2):127-32. doi: 10.1016/0165-2478(87)90043-5.
Destruction of articular cartilage is the hallmark of both inflammatory and degenerative arthritides. Since degradation of cartilage results in the release of proteoglycan (PG) monomers and fragments into the synovial fluid, the present study was initiated to determine whether hyaline cartilage PG can induce a cellular immune response. Nonimmune spleen cells obtained from A/St and C57Bl/6 mice were cultured with a crude extract of bovine nasal cartilage, a PG aggregate fraction, PG monomer or degraded PG monomer for varying time periods. Only intact PG monomer induced a proliferative response which peaked at day 2. The responding cell was a B-lymphocyte which did not require T-helper cell activity. Our results suggest that intact PG monomer is a polyclonal activator of B-lymphocytes.
关节软骨的破坏是炎性和退行性关节炎的标志。由于软骨降解会导致蛋白聚糖（PG）单体和片段释放到滑液中，因此开展了本研究以确定透明软骨PG是否能诱导细胞免疫反应。将从A/St和C57Bl/6小鼠获得的非免疫脾细胞与牛鼻软骨粗提物、PG聚集体组分、PG单体或降解的PG单体一起培养不同时间段。只有完整的PG单体诱导了增殖反应，该反应在第2天达到峰值。反应细胞是一种不需要T辅助细胞活性的B淋巴细胞。我们的结果表明，完整的PG单体是B淋巴细胞的多克隆激活剂。
