Faculty of Fisheries, Vietnam National University of Agriculture, Hanoi, Viet Nam.
Faculty of Fisheries, Vietnam National University of Agriculture, Hanoi, Viet Nam.
Parasitol Int. 2022 Apr;87:102536. doi: 10.1016/j.parint.2021.102536. Epub 2021 Dec 31.
Myxosporean parasites Kudoa spp. have been reported in several marine fish species worldwide. However, little is known about the contamination of these parasites in raw fish in Southeast Asia, where the consumption demand of uncooked fish is increasing. In 2019, the occurrence of several cases of raw yellowfin tuna (Thunnus albacares) obtained from retail shops with the presence of unknown white, nodular cysts within the musculature have raised public health concerns for the consumption of raw marine fish in Vietnam. Microscopic examination revealed numerous myxospores with the quadratic shape of the Kudoidae. Morphologically, stained spores detected in this study are suspected to Kudoa thunni. To confirm the suspected Kudoa species, further examination of the 18S small-subunit (SSU) was conducted and the results of nucleotide sequence analysis obtained from nodular cysts revealed 99.18-100% identity to that of Kudoa thunni sequences available in GenBank. Detection of K. thunni infection in tuna in Southeast Asia highlights the need for appropriate surveillance and control measures to ensure high quality standards and safety on raw fish production and consumption.
粘孢子虫 Kudoa spp. 已在世界范围内的几种海洋鱼类中被报道。然而，对于东南亚这些寄生虫在生鱼片中的污染情况知之甚少，因为东南亚对生鱼片的消费需求正在增加。2019 年，在零售商店购买的生黄鳍金枪鱼（Thunnus albacares）中出现了几例不明的白色、结节状囊肿存在于肌肉组织内，这引起了越南公众对食用生海洋鱼类的健康关注。显微镜检查显示大量具有 Kudoidae 形状的二分孢子。形态学上，本研究中检测到的染色孢子疑似为 Kudoa thunni。为了确认疑似 Kudoa 种，进一步对 18S 小亚基（SSU）进行了检查，从结节囊肿中获得的核苷酸序列分析结果与 GenBank 中可用的 Kudoa thunni 序列具有 99.18-100%的同一性。在东南亚金枪鱼中检测到 K. thunni 感染，突出表明需要采取适当的监测和控制措施，以确保生鱼片生产和消费的高标准和安全性。
