Zhang Meng, Ma Yamin, Luo Junyu, Ji Jichao, Gao Xueke, Wu Changcai, Zhu Xiangzhen, Wang Li, Zhang Kaixin, Li Dongyang, Wang Lisha, Niu Lin, Cui Jinjie
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Henan, Zhengzhou 450001, China; Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Ecotoxicol Environ Saf. 2022 Jan 15;230:113129. doi: 10.1016/j.ecoenv.2021.113129. Epub 2021 Dec 31.
The large-scale commercial cultivation of genetically modified (GM) cotton has brought significant economic and environmental benefits. However, GM crops must undergo strict environmental monitoring and long-term observation. An important natural enemy insect in cotton fields, Geocoris pallidipennis, can ingest the Bt protein expressed in GM cotton by feeding on herbivorous insects that feed on the cotton. However, the potential risk of GM cotton to G. pallidipennis is still unclear. We here evaluated the effects of Bt cotton expressing the Cry1Ac/1Ab protein on nymphs and adults G. pallidipennis. Cry1Ac protein was detected in the midgut of the cotton bollworm, Helicoverpa armigera, after it ingested Bt cotton, and in the midgut of G. pallidipennis nymphs and adults preying on Bt-fed H. armigera. However, the survival rate, growth, development, and fecundity of G. pallidipennis were not adversely affected. Furthermore, G. pallidipennis cadherins, and those genes related to detoxification, antioxidant activity, nutrient utilization, and immune function were not differentially expressed in response to Cry1Ac exposure. Finally, we showed that Cry1Ac could not bind to brush border membrane vesicles (BBMV) proteins in G. pallidipennis nymphs or adults. In summary, these results indicate that the potential negative effect of transgenic Cry1Ac/1Ab cotton on the insect redator G. pallidipennis is negligible.
转基因棉花的大规模商业化种植带来了显著的经济和环境效益。然而，转基因作物必须经过严格的环境监测和长期观察。棉田中的一种重要天敌昆虫——淡色姬蝽，可通过取食取食棉花的植食性昆虫来摄取转基因棉花中表达的Bt蛋白。然而，转基因棉花对淡色姬蝽的潜在风险仍不明确。我们在此评估了表达Cry1Ac/1Ab蛋白的Bt棉花对淡色姬蝽若虫和成虫的影响。棉铃虫取食Bt棉花后，在其肠道中检测到了Cry1Ac蛋白，在捕食取食Bt棉花的棉铃虫的淡色姬蝽若虫和成虫的肠道中也检测到了该蛋白。然而，淡色姬蝽的存活率、生长、发育和繁殖力并未受到不利影响。此外，淡色姬蝽的钙黏蛋白以及与解毒、抗氧化活性、营养利用和免疫功能相关的基因在接触Cry1Ac后并未发生差异表达。最后，我们发现Cry1Ac不能与淡色姬蝽若虫或成虫的刷状缘膜囊泡（BBMV）蛋白结合。总之，这些结果表明转基因Cry1Ac/1Ab棉花对捕食性昆虫淡色姬蝽的潜在负面影响可忽略不计。
