Justice D L, Rhodes R H, Tökés Z A
J Cell Biochem. 1987 Aug;34(4):227-38. doi: 10.1002/jcb.240340402.
The presence of alpha-1-antichymotrypsin, a serine proteinase inhibitor with a high affinity for cathepsin G, is demonstrated in the normal human central nervous system (CNS) by immunohistochemical techniques. Paraffin-embedded normal human CNS tissue from five adult, two fetal, one neonatal and three newborn autopsies were stained with monospecific rabbit antibodies to human alpha-1-antichymotrypsin using biotinylated goat anti-rabbit antibodies and an avidinbiotin-peroxidase complex. Positive immunostaining was seen in neurons and glial cells in the cerebral cortex, basal ganglia, hippocampus, cerebellum, brainstem, and spinal cord of the adults. The epithelium of the adult choroid plexus had the most intense staining in apical granular organelles corresponding in position to lysosomes or secretory granules. Ependymal cells, particularly those near the choroid plexus, were immunostained. The fetal CNS had no alpha-1-antichymotrypsin staining. Limited staining of choroid plexus, ependyma, and frontal lobe was found in the newborns. Immunostaining in the neonatal temporal lobe was only found in the choroid-plexus epithelium. These observations establish a widespread distribution of this proteinase inhibitor in the normal human CNS. Developmental regulation of this inhibitor in the human CNS is also indicated.
通过免疫组织化学技术在正常人类中枢神经系统（CNS）中证实了α1抗糜蛋白酶的存在，α1抗糜蛋白酶是一种对组织蛋白酶G具有高亲和力的丝氨酸蛋白酶抑制剂。使用生物素化的山羊抗兔抗体和抗生物素蛋白-生物素-过氧化物酶复合物，用针对人α1抗糜蛋白酶的单特异性兔抗体对来自五例成人、两例胎儿、一例新生儿和三例新生儿尸检的石蜡包埋正常人类CNS组织进行染色。在成人的大脑皮质、基底神经节、海马体、小脑、脑干和脊髓的神经元和神经胶质细胞中可见阳性免疫染色。成人脉络丛上皮在与溶酶体或分泌颗粒相对应的顶端颗粒细胞器中染色最强。室管膜细胞，特别是靠近脉络丛的那些细胞，被免疫染色。胎儿CNS没有α1抗糜蛋白酶染色。在新生儿中发现脉络丛、室管膜和额叶有有限的染色。在新生儿颞叶中，免疫染色仅见于脉络丛上皮。这些观察结果证实了这种蛋白酶抑制剂在正常人类CNS中的广泛分布。也表明了该抑制剂在人类CNS中的发育调节。
