Gresik E W, Maruyama S
J Gerontol. 1987 Sep;42(5):491-6. doi: 10.1093/geronj/42.5.491.
Effects of advancing age on responsiveness to triiodothyronine (T3) or dihydrotestosterone (DHT) by granular convoluted tubule (GCT) cells in submandibular glands were studied in C57BL/6NNia female mice of 4, 12, and 24 months of age. In untreated mice, absolute GCT volume and the frequency of cells strongly immunostained for EGF increased with age. Concentrations of EGF rose fourfold. Both T3 and DHT had inductive effects at each age: GCT cells were enlarged, contained more granules, and stained very strongly for EGF; EGF concentrations and the volume of GCTs also increased. However, the pattern of induction differed with age for the two hormones. After T3 treatment EGF concentration declined with age with respect to the degree of induction over same age control levels. By contrast, after DHT treatment the level of induction of EGF over control levels was the same at all three ages. The pattern of aging of GCT cells and their responsiveness to T3 or DHT in female mice differ from that previously reported for males.
在4、12和24月龄的C57BL/6NNia雌性小鼠中，研究了年龄增长对下颌下腺颗粒曲管（GCT）细胞对三碘甲状腺原氨酸（T3）或二氢睾酮（DHT）反应性的影响。在未经处理的小鼠中，GCT的绝对体积以及对表皮生长因子（EGF）免疫染色强烈的细胞频率随年龄增加。EGF的浓度增加了四倍。T3和DHT在各个年龄段均具有诱导作用：GCT细胞增大，含有更多颗粒，并且对EGF染色非常强烈；EGF浓度和GCT的体积也增加。然而，两种激素的诱导模式随年龄而异。T3处理后，相对于同年龄对照水平的诱导程度，EGF浓度随年龄下降。相比之下，DHT处理后，EGF在所有三个年龄段的诱导水平相对于对照水平均相同。雌性小鼠中GCT细胞的衰老模式及其对T3或DHT的反应性与先前报道的雄性小鼠不同。
