Hosoi K, Tanaka I, Ueha T
J Biochem. 1981 Jul;90(1):267-70. doi: 10.1093/oxfordjournals.jbchem.a133461.
The content of epidermal growth factor (EGF) as a high molecular weight complex (HMW-EGF) in the submandibular glands of mice was measured simply by a single radial immunodiffusion method. In female mice, the amount of HMW-EGF was increased 10-fold by tri-iodo-L-thyronine (T3) and 60-fold by 5 alpha-dihydrotestosterone (5 alpha-DHT). In mice with testicular feminization (Tfm), which are genetically deficient in androgen receptor, T3 but not 5 alpha-DHT increased the HMW-EGF from a non-detectable level to 5.4 +/- 0.94 micrograms/mg protein. It was concluded that EGF is also synthesized under the control of thyroid hormone in vivo, and that androgen was not involved in this induction of EGF by thyroid hormone.
采用单向免疫扩散法简单测定了小鼠颌下腺中作为高分子量复合物（HMW - EGF）的表皮生长因子（EGF）含量。在雌性小鼠中，三碘 - L - 甲状腺原氨酸（T3）使HMW - EGF含量增加10倍，5α - 双氢睾酮（5α - DHT）使其增加60倍。在雄激素受体基因缺陷的睾丸雌性化（Tfm）小鼠中，T3而非5α - DHT可使HMW - EGF从不可检测水平增加至5.4±0.94微克/毫克蛋白。得出的结论是，EGF在体内也受甲状腺激素调控合成，且雄激素不参与甲状腺激素对EGF的这种诱导作用。
