Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CP 26077, 05508-000 São Paulo, Brazil.
Department of Materials, School of Natural Sciences, Faculty of Science and Engineering and The Henry Royce Institute, Royce Hub Building, The University of Manchester, Manchester M13 9PL, United Kingdom.
Biointerphases. 2022 Jan 3;17(1):011001. doi: 10.1116/6.0001435.
Electroactive materials based on conductive polymers are promising options for tissue engineering and regenerative medicine applications. In the present work, the conducting copolymers of poly (3,4-ethylenedioxythiophene) and poly (d, l-lactic acid) (PEDOT-co-PDLLA) with PEDOT:PDLLA molar ratios of 1:50, 1:25, and 1:5 were synthesized and compared to the insulating macromonomer of EDOT-PDLLA as an experimental control. Bone marrow-derived human mesenchymal stem cells (hMSC-BM) were cultured on the copolymers and the macromonomer thin films inside a bioreactor that induced a capacitive electrical stimulation (CES) with an electric field of 100 mV/mm for 2 h per day for 21 days. Under CES, the copolymers exhibited good cell viability and promoted the differentiation from hMSC-BM to osteogenic lineages, revealed by higher mineralization mainly when the contents of conducting segments of PEDOT (i.e., copolymer with 1:25 and 1:5 PEDOT:PDLLA ratios) were increased. The results indicate that the intrinsic electrical conductivity of the substrates is an important key point for the effectiveness of the electric field generated by the CES, intending to promote the differentiation effect for bone cells.
基于导电聚合物的电活性材料是组织工程和再生医学应用的有前途的选择。在本工作中，合成了聚（3,4-亚乙基二氧噻吩）和聚（D，L-乳酸）（PEDOT-co-PDLLA）的导电共聚物，PEDOT:PDLLA 的摩尔比为 1:50、1:25 和 1:5，并与作为实验对照的绝缘大分子单体 EDOT-PDLLA 进行了比较。骨髓来源的人间充质干细胞（hMSC-BM）在共聚物和大分子单体薄膜上培养，在生物反应器内进行电容式电刺激（CES），每天施加 100 mV/mm 的电场 2 小时，共 21 天。在 CES 下，共聚物表现出良好的细胞活力，并促进 hMSC-BM 向成骨谱系的分化，主要通过增加导电段 PEDOT 的含量（即 PEDOT:PDLLA 比为 1:25 和 1:5 的共聚物）来提高矿化程度。结果表明，基底的固有电导率是 CES 产生的电场有效性的一个重要关键点，旨在促进骨细胞的分化效果。
