Hall Ruth M
School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.
Mob DNA. 2022 Jan 3;13(1):1. doi: 10.1186/s13100-021-00257-9.
The insertion sequence IS26 has long been known to play a major role in the recruitment of antibiotic resistance genes into the mobile resistance gene pool of Gram-negative bacteria and IS26 also plays a major role in their subsequent broad dissemination. Related IS, IS431/257 and IS1216 are important in the same roles in Gram positive bacteria. However, until recently the properties of IS26 movement that could potentially explain this ability had not been explored. A much needed insight has come from our recent demonstration that IS26 uses a novel targeted mechanism that is conservative. The targeted conservative mechanism is much more efficient than the known replicative mechanism, which is now more accurately called copy-in. A recent review "The IS6 family, a clinically important group of insertion sequences including IS26" by Varani, He, Siguier, Ross and Chandler published in Mobile DNA has substantially misrepresented the recent studies on the targeted conservative mechanism and at the same time incorrectly implied that any mechanism established for IS26 can be assumed to apply to a range of IS that are at best very distantly related. A few of the most important issues are examined in this comment. Readers are advised to consult the original literature to check facts before drawing firm conclusions.
插入序列IS26长期以来被认为在将抗生素抗性基因招募到革兰氏阴性菌的移动抗性基因库中起主要作用，并且IS26在其随后的广泛传播中也起主要作用。相关的插入序列IS431/257和IS1216在革兰氏阳性菌中在相同作用方面很重要。然而，直到最近，可能解释这种能力的IS26移动特性尚未得到探索。我们最近证明IS26使用一种新型的靶向保守机制，这带来了急需的见解。这种靶向保守机制比已知的复制机制（现在更准确地称为“复制入”）效率高得多。Varani、He、Siguier、Ross和Chandler最近在《移动DNA》上发表的一篇综述《IS6家族，包括IS26在内的临床上重要的插入序列组》严重歪曲了关于靶向保守机制的最新研究，同时错误地暗示为IS26建立的任何机制都可以假定适用于一系列充其量只是远缘相关的插入序列。本评论探讨了一些最重要的问题。建议读者在得出确凿结论之前查阅原始文献以核实事实。
