College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Department of Food Science, Foshan University, Foshan 528000, China; Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, Guangdong 350108, China.
Food Res Int. 2022 Jan;151:110847. doi: 10.1016/j.foodres.2021.110847. Epub 2021 Dec 2.
Photodynamic inactivation (PDI) is an effective alternative to traditional antibiotics to broadly kill bacteria. This study aimed to develop a potent PDI system by coupling calcinated melamine sponges (CMSs) with the Fenton reaction. The results showed that CMS calcined at 350 ℃ was successfully carbonized with intact and porous structures, and it possessed excellent hydrophilicity and photothermal conversion performance. When Fe was added and internalized, the Fenton reaction in which Fe reacted with HO in cells occurred to produce reactive oxygen species (ROS) (OH, OOH, etc.) and O, and notably, the O molecules could serve as a raw material to absorb the photothermal energy of CMS to generate highly reactive O. Under synergistic effects, CMS-350 coupled with Fe potently inactivated > 6 Log CFU/mL (>99.9999%) of Salmonella under 201.6 J/cm blue LED illumination by destroying Na/K-ATPase and Ca/Mg-ATPase, DNA synthesis-related enzymes, cell membranes, etc. Meanwhile, the composite photocatalyst was proven to be nontoxic and could inactivate Salmonella in various foods, including vegetables (Brassica chinensis L), eggs and fresh cucumber juice. As a result, CMS coupled with the Fenton reaction greatly improves the inactivation potency of PDI against harmful bacteria.
光动力灭活（PDI）是一种替代传统抗生素广泛杀死细菌的有效方法。本研究旨在通过将煅烧三聚氰胺海绵（CMS）与芬顿反应相结合来开发一种有效的 PDI 系统。结果表明，在 350℃下煅烧的 CMS 成功碳化，具有完整和多孔的结构，并且具有优异的亲水性和光热转换性能。当添加和内化 Fe 时，Fe 与细胞内的 HO 发生芬顿反应，产生活性氧物种（ROS）（OH、OOH 等）和 O，值得注意的是，O 分子可以作为一种原料吸收 CMS 的光热能量，产生高活性的 O。在协同作用下，CMS-350 在 201.6 J/cm 蓝色 LED 照射下，通过破坏 Na/K-ATP 酶和 Ca/Mg-ATP 酶、DNA 合成相关酶、细胞膜等，有效地将 >6 Log CFU/mL（>99.9999%）的沙门氏菌灭活。同时，证明复合光催化剂是无毒的，可以在各种食品中灭活沙门氏菌，包括蔬菜（白菜）、鸡蛋和新鲜黄瓜汁。因此，CMS 与芬顿反应的结合大大提高了 PDI 对有害细菌的灭活效力。
