Drews G
Pflugers Arch. 1987 Jul;409(3):251-7. doi: 10.1007/BF00583473.
The effects of chloramine-T, a substance which partially and irreversibly inhibits inactivation of the Na current (Wang 1984), on the on and off charge movement (Qon and Qoff) and on the relative position of the Qon-E and F-E curves were studied on voltage clamped frog nodes of Ranvier. The decrease of Qoff with increasing pulse duration which is seen in normal fibres was much less pronounced in chloramine-T-treated fibres, i.e. chloramine-T inhibited charge immobilization. Also, the decrease of the time constant tau off which normally accompanies the decrease of Qoff was absent in chloramine-T-treated fibres. A correlation (r2 = 0.80) between the relative tau off and the relative Qoff was observed in chloramine-T-treated fibres. The voltage dependence of the on charge movement, Qon-E, was compared with the voltage dependence of the fraction of open channels, F-E. In normal fibres, the F-E curve rose very steeply and crossed the normalized Qon-E curve so that F greater than Qon/Qon max for E greater than -40 mV. After chloramine-T treatment, the F-E curve rose less steeply and remained below the normalized Qon-E curve at all potentials. The effect of halazone was similar to that of chloramine-T but weaker. The observations about the Qon-E and F-E curve are compared with the findings on the squid giant axon.
在电压钳制的蛙类郎飞氏结上，研究了氯胺 - T（一种能部分且不可逆地抑制钠电流失活的物质，Wang，1984）对开启和关闭电荷移动（Qon和Qoff）以及Qon - E和F - E曲线相对位置的影响。在正常纤维中观察到的随着脉冲持续时间增加Qoff的降低，在经氯胺 - T处理的纤维中不太明显，即氯胺 - T抑制了电荷固定。此外，在经氯胺 - T处理的纤维中不存在通常伴随Qoff降低的时间常数tau off的降低。在经氯胺 - T处理的纤维中观察到相对tau off和相对Qoff之间的相关性（r2 = 0.80）。将开启电荷移动的电压依赖性Qon - E与开放通道分数的电压依赖性F - E进行了比较。在正常纤维中，F - E曲线上升非常陡峭，并与归一化的Qon - E曲线相交，使得对于E大于 - 40 mV，F大于Qon/Qon max。经氯胺 - T处理后，F - E曲线上升较平缓，并且在所有电位下都保持在归一化的Qon - E曲线下方。哈拉宗的作用与氯胺 - T相似但较弱。将关于Qon - E和F - E曲线的观察结果与在枪乌贼巨大轴突上的发现进行了比较。
