连续经尿道膀胱测压：一种评估成年雌性大鼠反射性下尿路功能的新方法。
Serial transurethral cystometry: A novel method for longitudinal evaluation of reflex lower urinary tract function in adult female rats.
机构信息
Health and Rehabilitation Sciences, School of Health Technology & Management, Stony Brook University, Stony Brook, New York, USA.
Temple University, Philadelphia, Pennsylvania, USA.
出版信息
Physiol Rep. 2022 Jan;10(1):e15131. doi: 10.14814/phy2.15131.
AIMS
The aim of the study is to develop a minimally invasive method for longitudinal evaluation of lower urinary tract function that allows for simultaneous measurements of bladder pressure and external urethral sphincter (EUS) electromyographic (EMG) activity.
METHODS
To evaluate the reliability of serial transurethral cystometry (STUC), rats (n = 12) underwent three sessions of STUC, one session a week for 3 weeks. During each session, rats were anesthetized with ketamine-xylazine (90 mg/kg and 10 mg/kg), and micturition reflex data were acquired using transurethral cystometry and percutaneous recording of EUS (EMG) activity during continuous infusion of saline into the bladder. The reliability and consistency of the STUC method were assessed using intra-class correlation (ICC) analysis and repeated measures ANOVA.
RESULTS
ICC values calculated from five successive events during the first micturition session indicate good to excellent reliability for measurements of peak bladder pressure, threshold bladder pressure, minimum bladder pressure, volume threshold, duration of EUS bursting, and number of EUS burst events. Across the three recording sessions no significant difference was observed in peak bladder pressure, threshold bladder pressure, minimum bladder pressure, volume threshold, number of EUS burst events, and duration of EUS bursting using repeated measures ANOVA.
CONCLUSION
Serial transurethral cystometry under ketamine-xylazine anesthesia with simultaneous percutaneous EUS EMG recording is a novel, reliable, accurate, and minimally invasive method for quantitative assessment of lower urinary tract (LUT) function in adult female rats over extended periods of time.
目的
本研究旨在开发一种微创方法，用于对下尿路功能进行纵向评估，该方法允许同时测量膀胱压力和尿道外括约肌（EUS）肌电图（EMG）活动。
方法
为了评估连续经尿道膀胱测压（STUC）的可靠性，12 只大鼠分 3 组，每周进行 1 次，共进行 3 周。在每次试验中，大鼠均用氯胺酮-甲苯噻嗪（90mg/kg 和 10mg/kg）麻醉，通过经尿道膀胱测压和对尿道外括约肌（EMG）活动进行连续的经皮记录，在向膀胱持续输注盐水的过程中获取排尿反射数据。使用组内相关系数（ICC）分析和重复测量方差分析评估 STUC 方法的可靠性和一致性。
结果
第一次排尿试验中连续 5 次事件的 ICC 值表明，测量膀胱压力峰值、膀胱压力阈值、最小膀胱压力、容积阈值、EUS 爆发持续时间和 EUS 爆发事件数具有良好到极好的可靠性。通过重复测量方差分析，在 3 个记录试验中，膀胱压力峰值、膀胱压力阈值、最小膀胱压力、容积阈值、EUS 爆发事件数和 EUS 爆发持续时间均无显著差异。
结论
在氯胺酮-甲苯噻嗪麻醉下进行连续经尿道膀胱测压，同时进行经皮 EUS EMG 记录，是一种新颖、可靠、准确且微创的方法，可用于在较长时间内对成年雌性大鼠的下尿路（LUT）功能进行定量评估。
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