Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Vector Borne Zoonotic Dis. 2022 Jan;22(1):48-54. doi: 10.1089/vbz.2021.0053. Epub 2022 Jan 3.
infection is endemic in countries along the Mekong River and certain of its tributaries in the lower Mekong basin, especially in Lao People's Democratic Republic and Cambodia. Diagnosis of schistosomiasis is crucial before treatment and epidemiological surveys before and/or after an intervention, such as a mass drug administration. A newly developed immunochromatographic test (ICT) for the diagnosis of schistosomiasis mekongi, based on antiparasite antibody detection in human sera, was evaluated. The schistosomiasis mekongiICT (ICT) strip was developed using somatic antigen from adult . In total, 209 serum samples were examined, including 14 from parasitologically proven schistosomiasis mekongi patients, 30 from schistosomiasis japonica patients, other parasitosis ( = 135), and healthy volunteers ( = 30) from areas not endemic for . Eleven schistosomiasis mekongi samples were positive according to the ICT, whereas all healthy control samples were negative. Crossreactions with paragonimiasis heterotremus, sparganosis, trichinellosis, and taeniasis saginata samples were observed at 2.4% (4/165). The diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 78.6% (95% confidence interval [CI] 49.2-95.3), 97.6% (95% CI 93.9-99.3), 73.3% (95% CI 44.9-92.2), 98.2% (95% CI 94.7-99.6), and 96.1% (95% CI 92.1-98.4), respectively. The ICT kit might be useful to assess the prevalence of disease before establishing transmission control and mass deworming campaigns in countries in the Mekong River subregion.
在湄公河流域及其下游的一些支流国家，如老挝人民民主共和国和柬埔寨，曼氏血吸虫病呈地方性流行。在进行治疗和在干预（如大规模药物治疗）前后进行流行病学调查之前，对血吸虫病进行诊断至关重要。本研究评估了一种新开发的曼氏血吸虫病免疫层析检测法（ICT），该方法基于人体血清中寄生虫抗体的检测。曼氏血吸虫病 ICT 条带是使用成虫的体抗原开发的。共检测了 209 份血清样本，包括 14 份寄生虫学证实的曼氏血吸虫病患者、30 份日本血吸虫病患者、其他寄生虫病（=135 例）和非曼氏血吸虫病流行地区的健康志愿者（=30 例）。根据 ICT，有 11 份曼氏血吸虫病样本呈阳性，而所有健康对照样本均为阴性。与并殖吸虫病、裂头蚴病、旋毛虫病和带绦虫病样本的交叉反应率为 2.4%（4/165）。诊断的敏感性、特异性、阳性预测值、阴性预测值和准确性分别为 78.6%（95%置信区间 49.2-95.3）、97.6%（95%置信区间 93.9-99.3）、73.3%（95%置信区间 44.9-92.2）、98.2%（95%置信区间 94.7-99.6）和 96.1%（95%置信区间 92.1-98.4）。该 ICT 试剂盒可能有助于评估湄公河流域国家在开展疾病传播控制和大规模驱虫运动之前的疾病流行情况。
