Thiangtrongjit Tipparat, Adisakwattana Poom, Limpanont Yanin, Nguitragool Wang, Chusongsang Phiraphol, Chusongsang Yupa, Kiangkoo Nuttapohn, Reamtong Onrapak
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Heliyon. 2024 Jul 30;10(15):e35439. doi: 10.1016/j.heliyon.2024.e35439. eCollection 2024 Aug 15.
Schistosomiasis caused by is one of the causative agents of human blood fluke infection in the lower Mekong River. Traditionally, the detection of egg morphology in stool samples has served as the prevailing method for diagnosing infection. Nonetheless, this approach exhibits low sensitivity, particularly in early infection detection. Urine has been extensively studied as a noninvasive clinical sample for diagnosing infectious diseases. Despite this, urine proteomic analysis of infection has been less investigated. This study aimed to characterize proteins and peptides present in mouse urine infected with both before infection and at intervals of 1, 2, 4, and 8 weeks post-infection using mass spectrometry-based proteomics. Proteomics analysis revealed 13 up- and only one down-regulated mouse protein consistently found across all time points. Additionally, two uncharacterized proteins were detected throughout the infection period. Using a peptidomics approach, we consistently identified two peptide sequences corresponding to collagen alpha-1(V) in mouse urine across all time points. These findings highlight the potential of these unique proteins, particularly the uncharacterized proteins and collagen alpha-1(V), as potential biomarkers for early detection of infection. Such insights could significantly advance diagnostic strategies for human Mekong schistosomiasis.
由[具体血吸虫种类未给出]引起的血吸虫病是湄公河下游地区人类感染血吸虫的病原体之一。传统上，通过检测粪便样本中的虫卵形态一直是诊断[具体血吸虫种类未给出]感染的主要方法。然而，这种方法灵敏度较低，尤其是在早期感染检测方面。尿液作为一种用于诊断传染病的非侵入性临床样本已得到广泛研究。尽管如此，针对[具体血吸虫种类未给出]感染的尿液蛋白质组学分析却较少被研究。本研究旨在利用基于质谱的蛋白质组学方法，对感染[具体血吸虫种类未给出]的小鼠在感染前以及感染后1周、2周、4周和8周时尿液中存在的蛋白质和肽进行表征。蛋白质组学分析显示，在所有时间点均一致发现13种上调和仅1种下调的小鼠蛋白质。此外，在整个感染期间检测到两种[具体血吸虫种类未给出]未表征的蛋白质。使用肽组学方法，我们在所有时间点均一致鉴定出小鼠尿液中与[具体血吸虫种类未给出]胶原蛋白α-1(V)相对应的两个肽序列。这些发现突出了这些独特蛋白质的潜力，特别是[具体血吸虫种类未给出]未表征的蛋白质和胶原蛋白α-1(V)，作为[具体血吸虫种类未给出]感染早期检测的潜在生物标志物。这些见解可能会显著推进人类湄公河血吸虫病的诊断策略。
