Simionescu L, Dumitru C, Zamfir-Grigorescu D, Dimitriu V, Câmpean G, Georgescu E, Stroescu V, Iosif C
Endocrinologie. 1987 Apr-Jun;25(2):101-6.
Taking into account the involvement of the measurement of osteocalcin, a protein-containing gamma-carboxyglutamic acid (Gla), in investigating the physiopathology of bone and calcium metabolism, a radioimmunoassay (RIA) method useful in human clinics was developed. The reagents for the RIA of human osteocalcin namely, the radioiodination preparation, the reference preparation and the antiserum were obtained starting from bovine bone on account of the structure similarities between bovine and human osteocalcin. The reagents were incubated at 4 degrees C for 48 h followed by the polyethylene-glycol (PEG) separation technique. The sensitivity of the osteocalcin RIA method is 2.0 ng/ml, the intraassay precision is 3-5% and the interassay precision, 8-12% (coeficient of variation). Osteocalcin was measured in the sera of 3 groups of performance sports girls under basal conditions and being of more or less similar ages: rowing girls (n = 10, mean age 16 yr 6 mos), gymnast girls (n = 13, mean age 14 yr 8 mos) and table tennis girls (n = 9, mean age 16 yr). The same samples were used for the measurement of myoglobin. Osteocalcin quantification showed similar levels in the rowing and the table tennis girls, i.e. 11.91 +/- 3.39 ng/ml (X +/- SD) and 9.50 +/- 4.15 ng/ml, respectively. By contrast, in the gymnast girls, the osteocalcin levels were about 4 times higher (44.31 +/- 7.27 ng/ml) than in the other two groups. Myoglobin measurements showed lower levels in the gymnast group, than in the rowing and the table tennis groups, the latter two having similar levels. The multiplicative increase of osteocalcin in the gymnast girls is interpreted and commented in relation to their age and sexual development.
考虑到骨钙素（一种含γ-羧基谷氨酸（Gla）的蛋白质）的测量在研究骨骼和钙代谢的生理病理学中的作用，开发了一种在人类临床中有用的放射免疫分析（RIA）方法。由于牛和人骨钙素之间的结构相似性，用于人骨钙素RIA的试剂，即放射性碘化制剂、参考制剂和抗血清，是从牛骨中获得的。将试剂在4℃下孵育48小时，然后采用聚乙二醇（PEG）分离技术。骨钙素RIA方法的灵敏度为2.0 ng/ml，批内精密度为3-5%，批间精密度为8-12%（变异系数）。在基础条件下，对3组年龄大致相似的竞技运动女孩的血清进行了骨钙素测量：赛艇女孩（n = 10，平均年龄16岁6个月）、体操女孩（n = 13，平均年龄14岁8个月）和乒乓球女孩（n = 9，平均年龄16岁）。相同的样本用于肌红蛋白的测量。骨钙素定量显示，赛艇女孩和乒乓球女孩的水平相似，分别为11.91±3.39 ng/ml（X±SD）和9.50±4.15 ng/ml。相比之下，体操女孩的骨钙素水平比其他两组高出约4倍（44.31±7.27 ng/ml）。肌红蛋白测量显示，体操组的水平低于赛艇组和乒乓球组，后两组水平相似。结合体操女孩的年龄和性发育情况，对她们骨钙素的成倍增加进行了解释和评论。
