Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA.
Deptartment of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, 29208, USA.
Curr Microbiol. 2022 Jan 4;79(2):45. doi: 10.1007/s00284-021-02742-z.
Caulobacter is a well-studied bacterial genus, but little is known about the plasmids that are found in some wild Caulobacter isolates. We used bioinformatic approaches to identify nine plasmids from seven different Caulobacter strains and grouped them based on their size and the similarity of their repABC, parAB, and mobAB genes. Protein pathway analysis of the genes on the K31p1 and K31p2 plasmids showed many metabolic pathways that would enhance the metabolic versatility of the host strain. In contrast, the CB4 plasmid contained 21 heavy metal resistance genes with the majority coding for proteins that enhance copper resistance. Growth assays of C. henricii CB4 demonstrated increased copper resistance and quantitative PCR showed an increase in the expression of eight heavy metal genes when induced with copper.
生丝微菌是一个研究得很好的细菌属，但是对于在一些野生生丝微菌分离物中发现的质粒知之甚少。我们使用生物信息学方法从七个不同的生丝微菌菌株中鉴定了九个质粒，并根据它们的大小和 repABC、parAB 和 mobAB 基因的相似性对它们进行分组。K31p1 和 K31p2 质粒上基因的蛋白途径分析显示了许多代谢途径，这将增强宿主菌株的代谢多样性。相比之下，CB4 质粒含有 21 个重金属抗性基因，其中大多数编码增强铜抗性的蛋白质。C. henricii CB4 的生长实验表明铜抗性增强，定量 PCR 显示在铜诱导时八个重金属基因的表达增加。
