环状RNA ciRs-126可能通过miR-21促进缺氧/复氧诱导的心脏损伤。
Circular RNA ciRs-126 promotes hypoxia/reoxygenation cardiac injury possibly through miR-21.
作者信息
Tan Changming, Li Jianming, Yuan Zhaoshun, Mu Yongxin
机构信息
Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, 410011, Changsha, Hunan, China.
Department of Medicine, University of California, La Jolla, CA, San Diego, USA.
出版信息
Thromb J. 2022 Jan 4;20(1):2. doi: 10.1186/s12959-021-00355-x.
BACKGROUND
This study aimed to analyze the role of circular RNA ciRs-126 in hypoxia/reoxygenation cardiac injury (H/R).
METHODS
Expression of ciRs-126 and miR-21 in plasma samples from patients with H/R and healthy controls was determined by RT-qPCR. Correlations were analyzed by linear regression. Overexpression of ciRs-126 and miR-21 was achieved in cardiomyocytes to explore their crosstalk. The roles of ciRs-126 and miR-21 in H/R-induced apoptosis of cardiomyocytes were analyzed using cell apoptosis assay.
RESULTS
CiRs-126 was upregulated and miR-21 was downregulated in H/R patients. They were inversely correlated across plasma samples from H/R patients. In H/R cardiomyocytes, ciRs-126 was upregulated and miR-21 was downregulated. In cardiomyocytes, ciRs-126 overexpression decreased miR-21 level and reduced the inhibitory effects of miR-21 overexpression on H/R-induced cell apoptosis.
CONCLUSIONS
Circular RNA ciRs-126 may suppress miR-21 expression to promote H/R cardiac injury.
背景
本研究旨在分析环状RNA ciRs-126在缺氧/复氧心脏损伤（H/R）中的作用。
方法
采用RT-qPCR法检测H/R患者和健康对照者血浆样本中ciRs-126和miR-21的表达。通过线性回归分析相关性。在心肌细胞中实现ciRs-126和miR-21的过表达以探索它们之间的相互作用。使用细胞凋亡检测分析ciRs-126和miR-21在H/R诱导的心肌细胞凋亡中的作用。
结果
H/R患者中ciRs-126上调，miR-21下调。在H/R患者的血浆样本中它们呈负相关。在H/R心肌细胞中，ciRs-126上调，miR-21下调。在心肌细胞中，ciRs-126过表达降低了miR-21水平，并减弱了miR-21过表达对H/R诱导的细胞凋亡的抑制作用。
结论
环状RNA ciRs-126可能通过抑制miR-21表达促进H/R心脏损伤。
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