急性心肌梗死后心肌纤维化中ceRNA（长链非编码RNA-微小RNA-信使RNA）调控网络的鉴定

Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction.

作者信息

Wang Shuo, Liu Yuying, Hu Xitian, Zhang Xiaolei, Xu Lei, Yang Yan, Wu Rubing, Wang Enmao, Lv Tianjie

机构信息

Department of Cardiovasology, Shijiazhuang People's Hospital, Shijiazhuang, People's Republic of China.

出版信息

Int J Gen Med. 2021 Dec 19;14:9977-9990. doi: 10.2147/IJGM.S329391. eCollection 2021.

PURPOSE

Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI.

MATERIALS AND METHODS

RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI.

RESULTS

Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results.

CONCLUSION

We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.

目的

急性心肌梗死（AMI）后的心肌纤维化（MF）最终会导致心力衰竭，这对人类生命构成严重威胁。本研究旨在挖掘与AMI后MF相关的关键生物标志物。

材料与方法

进行RNA测序以获取AMI及AMI后MF中差异表达的mRNA（DEmRNA）、miRNA（DEmiRNA）和lncRNA（DElncRNA）。

结果

在AMI及AMI后MF中鉴定出大量的DEmRNA、DEmiRNA和DElncRNA。获得了包含9个lncRNA-miRNA对和9个miRNA-mRNA对的ceRNA网络。在AMI中，所有候选标志物的表达模式总体上与我们的RNA测序结果一致；而在AMI后的MF中，除CENPB、JAK2和hsa-miR-197-3p外，qRT-PCR结果中其他标志物的表达模式与我们的RNA测序结果一致。