Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, BP 12, 23000, Annaba, Algeria.
Departamento de Biología, C/Darwin, 2, Universidad Autónoma de Madrid, ES-28049, Madrid, Spain.
Environ Sci Pollut Res Int. 2022 Apr;29(19):27624-27635. doi: 10.1007/s11356-021-17528-w. Epub 2022 Jan 4.
The cyanobacteria management in water bodies requires a deep knowledge of the community composition. Considering the reliable and thorough information provided by the polyphasic approach in cyanobacteria taxonomy, here we assess the cyanobacterial community structure of the Cheffia reservoir from Algeria. Cyanobacteria were identified on the basis of morphological traits and next-generation sequencing (NGS); toxins-related genes were localized in addition to the identification of toxins; temperature and nutrient level of water samples were also determined. The polyphasic approach was essential for cyanobacteria investigation; 28 genera were identified through 16S rRNA metabarcoding with the dominance of taxa from Microcystis (34.2%), Aphanizomenon (20.1%), and Planktothrix (20.0%), and morphological analysis revealed the association in this water body of five species within the genus Microcystis: M. aeruginosa, M. novacekii, M. panniformis, M. ichthyoblabe, and M. flos-aquae. The presence of mcyE genotypes was detected; moreover, HPLC-PDA and LC-ESI-MS/MS revealed the production of microcystin-LR. Results obtained in our study are very important since this ecosystem is used for water supply and irrigation; as a consequence, a good water management plan is essential.
水体中蓝藻的管理需要深入了解其群落组成。鉴于多相方法在蓝藻分类学中提供了可靠和详尽的信息，我们在此评估了来自阿尔及利亚 Cheffia 水库的蓝藻群落结构。蓝藻是基于形态特征和下一代测序 (NGS) 进行鉴定的；此外，还定位了毒素相关基因，并鉴定了毒素；还测定了水样的温度和营养水平。多相方法对于蓝藻调查至关重要；通过 16S rRNA 宏条形码鉴定出 28 个属，其中以微囊藻属（34.2%）、鱼腥藻属（20.1%）和束丝藻属（20.0%）为主导，形态学分析表明在该水体中存在五种微囊藻属的物种：铜绿微囊藻、新鱼腥藻、束丝藻、鱼腥藻和水花束丝藻。检测到 mcyE 基因型的存在；此外，HPLC-PDA 和 LC-ESI-MS/MS 显示微囊藻毒素-LR 的产生。我们的研究结果非常重要，因为这个生态系统用于供水和灌溉；因此，制定良好的水资源管理计划至关重要。
