Jones P D, Ada G L
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra.
Cell Immunol. 1987 Oct 1;109(1):53-64. doi: 10.1016/0008-8749(87)90291-7.
Influenza virus-specific antibody-secreting cells (ASCs), enumerated using an ELISA-plaque assay, were found in the lung and spleen up to 18 months after primary murine influenza infection. The number of ASCs generated in stimulated lung and spleen cell cultures increased 50- to 200-fold after influenza infection. Whereas the level of response did not change in spleen cell cultures up to 18 months after infection, there was a gradual reduction in ASCs in lung cell cultures obtained more than 6 months after infection, predominantly due to a reduction in B memory cells. Homotypic re-infection increased ASCs in the lung only, whereas B-cell memory increased in both the lung and spleen. Although ASCs increased in both the lung and spleen after heterotypic challenge, ASCs and B-cell memory specific for the original subtype were not increased.
利用酶联免疫吸附斑点试验（ELISA-斑点试验）计数发现，在原发性小鼠流感感染后的18个月内，肺和脾脏中存在流感病毒特异性抗体分泌细胞（ASC）。流感感染后，在受刺激的肺和脾细胞培养物中产生的ASC数量增加了50至200倍。感染后18个月内，脾细胞培养物中的反应水平没有变化，而在感染6个月后获得的肺细胞培养物中，ASC逐渐减少，主要是由于B记忆细胞减少。同型再次感染仅增加了肺中的ASC，而肺和脾中的B细胞记忆均增加。尽管异型攻击后肺和脾中的ASC均增加，但针对原始亚型的ASC和B细胞记忆并未增加。
