Walker K B, Potter J M, House A K
University Department of Surgery, Queen Elizabeth II Medical Centre, Nedlands, Perth, Western Australia.
Clin Exp Immunol. 1987 Apr;68(1):162-7.
In this study interleukin 2 (IL-2) synthesis by human lymphocytes in the presence and absence of prednisolone in a group of normal subjects has been assessed. An association between suppression in vitro of induced phytohaemagglutinin-blastogenesis by prednisolone and synthesis of IL-2 was found. Those subjects whose lymphocytes are identified as steroid-resistant have significantly higher IL-2 activity in the supernatants of both steroid and non-steroid treated lymphocyte cultures than steroid sensitive subjects. The addition of exogenous IL-2 was found to ablate the suppressive effects of steroids on lymphocyte blastogenesis. These results suggest that significantly greater activity of IL-2 in the culture supernatants of steroid resistant subjects may represent a mechanism for glucocorticoid resistance in vitro and help explain the relationship between increased loss of grafts and steroid resistance in renal allograft recipients.
在本研究中，评估了一组正常受试者在存在和不存在泼尼松龙的情况下人淋巴细胞白细胞介素2（IL-2）的合成。发现泼尼松龙对诱导的植物血凝素细胞增殖的体外抑制与IL-2的合成之间存在关联。那些淋巴细胞被鉴定为对类固醇耐药的受试者，其类固醇和非类固醇处理的淋巴细胞培养上清液中的IL-2活性明显高于类固醇敏感受试者。发现添加外源性IL-2可消除类固醇对淋巴细胞增殖的抑制作用。这些结果表明，类固醇耐药受试者培养上清液中IL-2活性显著更高可能代表体外糖皮质激素抵抗的一种机制，并有助于解释肾移植受者移植物丢失增加与类固醇抵抗之间的关系。
