Kroukamp Heinrich, Peng Kai, Paulsen Ian T, den Haan Riaan
Department of Molecular Sciences, ARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Biomolecular Discovery and Design Research Centre, Macquarie University, Sydney, NSW, Australia.
Methods Mol Biol. 2022;2378:19-30. doi: 10.1007/978-1-0716-1732-8_2.
The unfolded protein response (UPR) is a highly conserved protein quality control mechanism of eukaryotic cells. Aberrations in this response have been linked to several human diseases, including retinitis pigmentosa and several cancers, and have been shown to have a drastic impact on recombinant protein yields in fungal, insect, and mammalian cell lines. Here, we describe the use of in vivo biosensors to measure and characterize this dynamic cellular response, specifically for detecting the UPR induced by protein overproduction stress in the model cell factory Saccharomyces cerevisiae.
未折叠蛋白反应（UPR）是真核细胞中一种高度保守的蛋白质质量控制机制。这种反应的异常与多种人类疾病有关，包括色素性视网膜炎和几种癌症，并且已被证明对真菌、昆虫和哺乳动物细胞系中的重组蛋白产量有重大影响。在此，我们描述了使用体内生物传感器来测量和表征这种动态细胞反应，特别是用于检测模式细胞工厂酿酒酵母中蛋白质过量生产应激诱导的未折叠蛋白反应。
