Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
Vet Microbiol. 2022 Feb;265:109327. doi: 10.1016/j.vetmic.2021.109327. Epub 2021 Dec 30.
Pseudorabies is caused by pseudorabies virus (PRV), a member of the Herpesvirus family, and has caused tremendous damage to the pig industry. Protein unique lone 16 (pUL16) is a conserved envelope protein in all herpesviruses, that is known to play an important role in several aspects, including virus diffusion in cells and virulence in mice. It has been shown that the pUL16 can interact with the virus proteins UL11, UL49, UL21, gD, and gE. However, the research to date on pUL16 has only focused on etiology, without discussing the possible cellular pathways involved in PRV infection. Leucine-rich PPR motif-containing protein (LRPPRC) is a multifunctional cellular protein that participates in various cellular processes, such as RNA processing, splicing, stabilization, editing, translation, and energy metabolism. This was the first caspase-independent apoptosis protein to be identified. In this study, immune precipitation and mass spectrometry was performed to define the function of the pUL16 in PRV infection to study the possible cellular pathways in which pUL16 may participate. It was found that LRPRRC could interact with PRV pUL16, which may indicate that UL16 is involved in a redox reaction or cellular apoptosis. This is the first study of the interaction between pUL16 and host proteins, which has positive significance to gain a further understanding of the pUL16.
伪狂犬病是由伪狂犬病病毒（PRV）引起的，伪狂犬病病毒是疱疹病毒科的一员，它给养猪业造成了巨大的损失。蛋白独特孤 16（pUL16）是所有疱疹病毒中保守的包膜蛋白，已知它在几个方面发挥着重要作用，包括病毒在细胞内的扩散和在小鼠中的毒力。已经表明，pUL16 可以与病毒蛋白 UL11、UL49、UL21、gD 和 gE 相互作用。然而，迄今为止对 pUL16 的研究仅集中在病因学上，没有讨论 PRV 感染中可能涉及的细胞途径。富含亮氨酸的 PPR 基序蛋白（LRPPRC）是一种多功能的细胞蛋白，参与多种细胞过程，如 RNA 加工、剪接、稳定、编辑、翻译和能量代谢。这是第一个被鉴定的无半胱氨酸蛋白酶依赖性细胞凋亡蛋白。在这项研究中，通过免疫沉淀和质谱分析来定义 pUL16 在 PRV 感染中的功能，以研究 pUL16 可能参与的可能的细胞途径。结果发现 LRPPRC 可以与 PRV pUL16 相互作用，这可能表明 UL16 参与了氧化还原反应或细胞凋亡。这是首次研究 pUL16 与宿主蛋白之间的相互作用，这对进一步了解 pUL16 具有积极意义。
