Departamento de Química, Universidade Estadual de Maringá (UEM), Maringá, Brazil.
Programa de Pós-Graduação em Ciência de Alimentos, Universidade Estadual de Maringá (UEM), Maringá, Brazil.
Int J Cosmet Sci. 2022 Feb;44(1):131-141. doi: 10.1111/ics.12762. Epub 2022 Feb 10.
This study aimed to develop and validate a rapid, simple, accurate and precise analytical method for the quantification of L-AA in vitamin C serums. Moreover, the developed method was further applied to determine L-AA in eight different brands of vitamin C serums. A complementary study was also carried out to evaluate the stability of L-AA in the vitamin C serum samples after 15, 30, 45 and 60 days of storage at ambient temperature (15-35°C).
Ultra-high-performance liquid chromatography-tandem mass spectrometry was applied.
Quantitative analyses were performed with a total chromatographic run time of 1.5 min by matrix-matched calibration, and the analytical curve was linear over the range of 1-1700 µg L with a correlation coefficient of 0.9998. The limits of detection (LOD) and quantification (LOQ) were 0.3 and 1.0 µg L , respectively. Intra- and inter-assay precisions, expressed in terms of relative standard deviation, ranged from 0.3% and 2.2%, respectively, and recoveries in concentration levels of 1 and 5 µg L were 103.9% and 101.2%, respectively. The proposed analytical method was successfully applied to determine the L-AA content in eight commercial vitamin C serum samples. The stability of the target analyte in samples stored at ambient temperature (15-35°C) was evaluated throughout 60 days with a 15-day interval between analyses. At 0 days, L-AA content in samples ranged from 1.05 to 169.91 mg L , which decreases over time.
The proposed method could be powerful in routine analyses to ensure the quantification of L-AA in vitamin C serums since it proved to be a simple, reliable, fast, precise, accurate and sensitive analytical method.
本研究旨在开发和验证一种快速、简单、准确和精密的分析方法，用于定量测定维生素 C 血清中的 L-AA。此外，还进一步应用该方法测定了 8 种不同品牌的维生素 C 血清中的 L-AA。还进行了一项补充研究，以评估在环境温度（15-35°C）下储存 15、30、45 和 60 天后，L-AA 在维生素 C 血清样品中的稳定性。
应用超高效液相色谱-串联质谱法。
通过基质匹配校准进行定量分析，总色谱运行时间为 1.5 分钟，分析曲线在 1-1700 µg·L-1 范围内呈线性，相关系数为 0.9998。检测限（LOD）和定量限（LOQ）分别为 0.3 和 1.0 µg·L-1。日内和日间精密度以相对标准偏差表示，分别为 0.3%和 2.2%，在 1 和 5 µg·L-1 浓度水平的回收率分别为 103.9%和 101.2%。该分析方法成功应用于测定 8 种市售维生素 C 血清样品中的 L-AA 含量。通过 15 天的分析间隔，在环境温度（15-35°C）下评估了目标分析物在样品中的稳定性，持续 60 天。在 0 天，样品中 L-AA 含量在 1.05 至 169.91 mg·L-1 之间，随着时间的推移而减少。
该方法简单、可靠、快速、精确、准确且灵敏，可用于常规分析，以确保维生素 C 血清中 L-AA 的定量。
