Yang Jierui, Chen Benqi, Qiu Huiting, Meng Shuang, Yang Yunhui
College of Chemistry and Chemical Engineering, Yunnan Normal University Kunming China 650500
RSC Adv. 2023 Sep 1;13(37):26102-26110. doi: 10.1039/d3ra04202b. eCollection 2023 Aug 29.
Ascorbic acid is a nutritional small molecule essential to human life activities and health, playing a vital role in many physiological processes. Fresh fruits and beverages can provide abundant AA to maintain human metabolic balance. Therefore, it is of great significance to develop a nanomaterial with superior nanozyme activity for rapid and convenient detection of ascorbic acid (AA) in fruits and beverages. Herein, a dual-signal sensing platform based on UV-vis absorption and test strip chromaticity for the quantitative determination of AA is presented. The sensing platform is based on the horseradish peroxidase-like activity of NiVO nanoflowers, which catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide to the blue oxide TMB (ox TMB). The ox TMB produced by the oxidation has a characteristic absorption peak at 650 nm. In the presence of AA, the blue ox TMB is reduced to colorless TMB, and the quantitative detection of AA can be achieved by detecting the decrease in intensity of the absorption peak by UV-Vis spectrophotometry. Under the optimal experimental conditions, the sensing platform exhibited excellent sensitivity and selectivity. A wide linear range of 0.1 μM to 40 μM with a detection limit of 0.032 μM was obtained. The linear equation is Δ = 0.02513 + 0.1164 with a correlation coefficient of 0.9979. It showed excellent properties in the detection of real samples of fruit juices and beverages, meanwhile, a method for the rapid detection of AA based on chromaticity change of test strips was constructed with high sensitivity and convenience. The linearity range for the ascorbic acid was 1-50 μM with LOD of 0.42 μM. The developed sensing platform has the capability to quickly and accurately detect ascorbic acid (AA) in fresh fruits and beverages. This proposed method offers a new and promising approach for the rapid and cost-effective detection of ascorbic acid, which has a wide range of potential applications.
抗坏血酸是一种对人类生命活动和健康至关重要的营养小分子，在许多生理过程中发挥着关键作用。新鲜水果和饮料可以提供丰富的抗坏血酸以维持人体代谢平衡。因此，开发一种具有优异纳米酶活性的纳米材料用于快速便捷地检测水果和饮料中的抗坏血酸（AA）具有重要意义。在此，提出了一种基于紫外可见吸收和试纸条色度的双信号传感平台用于抗坏血酸的定量测定。该传感平台基于NiVO纳米花的辣根过氧化物酶样活性，其催化过氧化氢将3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色氧化物TMB（ox TMB）。氧化产生的ox TMB在650 nm处有特征吸收峰。在抗坏血酸存在的情况下，蓝色的ox TMB被还原为无色的TMB，通过紫外可见分光光度法检测吸收峰强度的降低可实现抗坏血酸的定量检测。在最佳实验条件下，该传感平台表现出优异的灵敏度和选择性。获得了0.1 μM至40 μM的宽线性范围，检测限为0.032 μM。线性方程为Δ = 0.02513 + 0.1164，相关系数为0.9979。它在果汁和饮料实际样品检测中表现出优异性能，同时构建了一种基于试纸条色度变化的高灵敏度、便捷的抗坏血酸快速检测方法。抗坏血酸的线性范围为1 - 50 μM，检测限为0.42 μM。所开发的传感平台有能力快速准确地检测新鲜水果和饮料中的抗坏血酸（AA）。该方法为抗坏血酸的快速、低成本检测提供了一种新的且有前景的途径，具有广泛的潜在应用。
