Los G, De Weger R A, Moberts R M, Van Loveren H, Sakkers R J, Den Otter W
Pathologisc Instituut, Department of Experimental Pathology, Utrecht, The Netherlands.
Immunology. 1987 Sep;62(1):89-95.
In different murine systems, delayed-type hypersensitivity (DTH) swelling responses at 24-48 hr after antigen challenge were preceded by an early 2-hr swelling response. The 24-hr DTH response is thought to depend on this early (DTH-initiating) hypersensitivity response. In this paper we show that in the syngeneic DBA/2-SL2 murine tumour system only an early 2-hr swelling response can be evoked. This early hypersensitivity response was tumour specific and serotonin dependent. The early hypersensitivity response in contact hypersensitivity has been ascribed to antigen-specific T-cell factors. To test whether similar T-cell factors were involved in the early hypersensitivity response in this syngeneic tumour system, we have transferred lymph node, spleen lymphocytes and serum from immunized mice into naive recipients. The serum was fractionated in two fractions, a 50,000-80,000 MW fraction, and a 120,000-190,000 MW fraction. In recipients of lymphocytes, total serum and the 50,000-80,000 MW fraction of the serum, an early hypersensitivity response can be evoked. So, these data suggest the involvement of specific T-cell factors in the development of an early hypersensitivity response against syngeneic tumour cells. Despite the development of an early (DTH initiating) hypersensitivity swelling response these immunized animals cannot develop a classical 24-hr swelling response. This absence of the 24-hr response in the presence of the 2-hr response is discussed in relation to the frequently observed immune suppression in tumour-bearing mice.
在不同的小鼠系统中，抗原攻击后24 - 48小时的迟发型超敏反应（DTH）肿胀反应之前会出现一个早期的2小时肿胀反应。24小时的DTH反应被认为依赖于这种早期（引发DTH的）超敏反应。在本文中我们表明，在同基因的DBA/2 - SL2小鼠肿瘤系统中，只能诱发早期的2小时肿胀反应。这种早期超敏反应具有肿瘤特异性且依赖于血清素。接触性超敏反应中的早期超敏反应归因于抗原特异性T细胞因子。为了测试在这个同基因肿瘤系统的早期超敏反应中是否涉及类似的T细胞因子，我们将免疫小鼠的淋巴结、脾淋巴细胞和血清转移到未免疫的受体小鼠体内。血清被分离成两个部分，一个50,000 - 80,000分子量的部分和一个120,000 - 190,000分子量的部分。在接受淋巴细胞、全血清以及血清50,000 - 80,000分子量部分的受体小鼠中，可以诱发早期超敏反应。所以，这些数据表明特异性T细胞因子参与了针对同基因肿瘤细胞的早期超敏反应的发展。尽管出现了早期（引发DTH的）超敏肿胀反应，但这些免疫小鼠无法产生典型的24小时肿胀反应。在存在2小时反应的情况下缺乏24小时反应的现象，结合在荷瘤小鼠中经常观察到的免疫抑制进行了讨论。
