Bluestone J A, Lopez C
Cancer Invest. 1983;1(1):5-13. doi: 10.3109/07357908309040928.
A functionally suppressive antigen-driven subpopulation of macrophages has been demonstrated in mice-bearing tumors produced by MSV-transformed BALB/3T3 cells. The suppressor macrophages depressed markedly both allogeneic and syngeneic mixed leukocyte culture responses and functioned even when assayed at low concentrations, suggesting that high concentrations of normal macrophages were not responsible for the suppression. Studies also showed that suppressor macrophages acted to suppress the proliferation of hyperreactive Lyt 1+,2- cells found in the splenic cell suspensions of tumor-bearing mice after the removal of the adherent suppressor cells. Finally, the suppressive activity of macrophages induced in tumorous mice could be augmented by KiMSV or MMSV but not by MuLV antigen. These results suggest that suppression might be a direct response to transformation-associated determinants expressed on the tumor cells as well as on the MSV virions or the result of cellular interactions between suppressor macrophages and other antigen-specific spleen cell populations.
在携带由MSV转化的BALB/3T3细胞产生的肿瘤的小鼠中，已证明存在一种功能上具有抑制作用的抗原驱动的巨噬细胞亚群。抑制性巨噬细胞显著降低了同种异体和同基因混合白细胞培养反应，即使在低浓度下检测也能发挥作用，这表明高浓度的正常巨噬细胞并非抑制作用的原因。研究还表明，在去除黏附性抑制细胞后，抑制性巨噬细胞会抑制荷瘤小鼠脾细胞悬液中发现的高反应性Lyt 1 +,2 - 细胞的增殖。最后，肿瘤小鼠中诱导的巨噬细胞的抑制活性可被KiMSV或MMSV增强，但不能被MuLV抗原增强。这些结果表明，抑制作用可能是对肿瘤细胞以及MSV病毒粒子上表达的转化相关决定簇的直接反应，或者是抑制性巨噬细胞与其他抗原特异性脾细胞群体之间细胞相互作用的结果。
