Michalek S M, Gregory R L, Harmon C C, Katz J, Richardson G J, Hilton T, Filler S J, McGhee J R
Department of Microbiology, University of Alabama at Birmingham 35294.
Infect Immun. 1987 Oct;55(10):2341-7. doi: 10.1128/iai.55.10.2341-2347.1987.
A multivalent vaccine consisting of whole cell antigens of seven strains, representing four serotypes (b, c, d and g), of mutans streptococci was used to hyperimmunize a group of cows. Serum samples from these animals contained immunoglobulin G1 (IgG1) antibody activity to seven serotypes (a to g) of mutans streptococci. Whey obtained from the animal with the highest serum antibody activity, which also contained high levels of IgG1 antibody, was used in passive caries immunity studies. Gnotobiotic rats monoinfected with Streptococcus mutans MT8148 serotype c or Streptococcus sobrinus OMZ176 (d) or 6715 (g) and provided a caries-promoting diet containing immune whey had lower plaque scores, numbers of streptococci in plaque, and degree of caries activity than similarly infected animals given a diet containing control whey obtained from nonimmunized cows. To establish the nature of the protective component(s) present in the immune whey, an ultrafiltrate fraction of the whey was prepared. This preparation contained higher levels of IgG1 anti-S. mutans antibody activity than the immune whey. Rats monoinfected with S. mutans MT8148 and provided with a diet supplemented with 0.1% of this fraction exhibited a degree of caries protection similar to that seen in animals provided a diet containing 100% immune whey. In fact, a diet containing as little as 0.01% of the ultrafiltrate fraction gave some degree of protection against oral S. mutans infection. The active component in the immune whey was the IgG1 anti-S. mutans antibody, since rats monoinfected with S. mutans MT8148 and provided a diet supplemented with purified immune whey IgG1 had significantly reduced plaque scores, numbers of S. mutans in plaque, and caries activity compared with control animals. Prior adsorption of the IgG fraction with killed S. mutans MT8148 whole cells removed antibody activity and abrogated caries protection.
一种多价疫苗由七种菌株的全细胞抗原组成，这些菌株代表变形链球菌的四种血清型（b、c、d和g），用于对一组奶牛进行超免疫。这些动物的血清样本含有针对变形链球菌七种血清型（a至g）的免疫球蛋白G1（IgG1）抗体活性。从血清抗体活性最高且IgG1抗体水平也很高的动物中获得的乳清，被用于被动龋齿免疫研究。单感染变形链球菌MT8148血清型c、嗜酸性链球菌OMZ176（d）或6715（g）并给予含免疫乳清的致龋饮食的无菌大鼠，与给予含未免疫奶牛的对照乳清饮食的类似感染动物相比，菌斑评分、菌斑中链球菌数量和龋齿活动程度更低。为了确定免疫乳清中存在的保护成分的性质，制备了乳清的超滤部分。该制剂比免疫乳清含有更高水平的抗变形链球菌IgG1抗体活性。单感染变形链球菌MT8148并给予补充有0.1%该部分的饮食的大鼠，其龋齿保护程度与给予含100%免疫乳清饮食的动物相似。事实上，含有低至0.01%超滤部分的饮食对口腔变形链球菌感染有一定程度的保护作用。免疫乳清中的活性成分是抗变形链球菌IgG1抗体，因为单感染变形链球菌MT8148并给予补充有纯化免疫乳清IgG1的饮食的大鼠，与对照动物相比，菌斑评分、菌斑中变形链球菌数量和龋齿活动显著降低。用灭活的变形链球菌MT8148全细胞预先吸附IgG部分可去除抗体活性并消除龋齿保护作用。
