Ward M M, Ward R E, Huang J H, Kohler H
Department of Molecular Immunology, Roswell Park Memorial Institute, New York State Department of Health, Buffalo 14263.
J Immunol. 1987 Oct 15;139(8):2775-80.
An analysis of nominal vs idiotope antigen-induced B cell precursors was performed in A/St mice. With the use of the splenic fragment culture system, the quantity and quality of B cell precursors responding to two anti-idiotope carrier antigens (4C11 hemocyanin and F6 hemocyanin) and nominal antigen (phosphorylcholine-hemocyanin) were compared. In addition, the effect of priming with anti-idiotope-carrier antigens on B cell precursors responding to phosphorylcholine-hemocyanin was determined. We found that one anti-idiotope-carrier antigen, 4C11-hemocyanin, and phosphorylcholine-hemocyanin stimulated similar subpopulations of primary B cells. However, the B cell population stimulated by F6-hemocyanin, the other anti-idiotype complex, was distinct. Furthermore, priming with certain idiotope antigens can direct the phosphorylcholine-hemocyanin response into the expression of idiotypes that may be the most effective in protective immunity. Our results provide essential information for the rational design of idiotype vaccines by clarifying the dynamic relationship of the B cell precursor repertoire with the in vivo antibody response in the response to nominal and idiotope antigens.
在A/St小鼠中对名义抗原与独特型抗原诱导的B细胞前体进行了分析。利用脾细胞片段培养系统，比较了对两种抗独特型载体抗原（4C11血蓝蛋白和F6血蓝蛋白）和名义抗原（磷酸胆碱 - 血蓝蛋白）作出反应的B细胞前体的数量和质量。此外，还确定了用抗独特型载体抗原进行启动对磷酸胆碱 - 血蓝蛋白反应的B细胞前体的影响。我们发现一种抗独特型载体抗原4C11 - 血蓝蛋白和磷酸胆碱 - 血蓝蛋白刺激的初级B细胞亚群相似。然而，另一种抗独特型复合物F6 - 血蓝蛋白刺激的B细胞群体是不同的。此外，用某些独特型抗原进行启动可将磷酸胆碱 - 血蓝蛋白反应导向可能在保护性免疫中最有效的独特型表达。我们的结果通过阐明在对名义抗原和独特型抗原的反应中B细胞前体库与体内抗体反应的动态关系，为独特型疫苗的合理设计提供了重要信息。
