Paque R E, Miller R, Thomas V
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.
Infect Immun. 1990 Mar;58(3):680-6. doi: 10.1128/iai.58.3.680-686.1990.
Polyclonal anti-idiotypic antibodies (anti-Ids)(fim) developed against idiotypes on antibodies (Ab-1s) that specifically bind structural, organelle fimbrial proteins of Escherichia coli were able to modulate immune function in anti-Id(fim)-immunized mice. Proliferation or suppression of splenic lymphoid cell responses by polyclonal anti-Ids in tissue culture appeared to be dose dependent. Anti-Ids were able to induce a dose-dependent T-cell-mediated immunity specific for type 1 fimbrial antigen(s) in immunized animals when assessed in vitro, but they failed to elicit in vivo positive ear-swelling skin reactions. Anti-Ids were unable to induce protective immunity against an in vivo infectious challenge with E. coli in anti-Id-immunized adult animals, but they stimulated a specific, secondary antibody response in anti-Id-challenged mice. Anti-Ids stimulated the development of anti-anti-Ids (Ab-3s) specifically binding a fimbrial antigen(s) and revealed the presence of antibody idiotypes binding E. coli adhesin proteins in the 27- to 29-kilodalton range. Results suggest discrete, but subtle, immunomodulatory effects of the anti-Ids and potential vaccinoid properties capable of stimulating a specific humoral and cellular response in vivo.
针对特异性结合大肠杆菌结构、细胞器菌毛蛋白的抗体（Ab-1s）上的独特型而产生的多克隆抗独特型抗体（抗-Id）（fim），能够调节抗-Id（fim）免疫小鼠的免疫功能。在组织培养中，多克隆抗-Id对脾淋巴细胞反应的增殖或抑制似乎呈剂量依赖性。在体外评估时，抗-Id能够在免疫动物中诱导针对1型菌毛抗原的剂量依赖性T细胞介导的免疫，但它们未能在体内引发阳性耳部肿胀皮肤反应。抗-Id在抗-Id免疫的成年动物中不能诱导针对大肠杆菌体内感染攻击的保护性免疫，但它们在抗-Id攻击的小鼠中刺激了特异性的二次抗体反应。抗-Id刺激了特异性结合菌毛抗原的抗抗-Id（Ab-3s）的产生，并揭示了在27至29千道尔顿范围内存在结合大肠杆菌粘附蛋白的抗体独特型。结果表明抗-Id具有离散但微妙的免疫调节作用，以及能够在体内刺激特异性体液和细胞反应的潜在类疫苗特性。
