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[温度对序批式生物膜反应器中厌氧氨氧化过程的影响:脱氮性能与细菌群落]

[Effect of Temperature on ANAMMOX Process in Sequencing Batch Biofilm Reactors: Nitrogen Removal Performance and Bacterial Community].

作者信息

Wu Shan, Wang Shu-Ya, Wang Fen, Ji Min

机构信息

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.

Ji'nan Urban Construction Group Co., Ltd., Ji'nan 250031, China.

出版信息

Huan Jing Ke Xue. 2022 Jan 8;43(1):416-423. doi: 10.13227/j.hjkx.202105280.

DOI:10.13227/j.hjkx.202105280
PMID:34989526
Abstract

For the ambient and low-temperature operation of the anaerobic ammonium oxidation (ANAMMOX) process, sequence batch biofilm reactors (SBBR) with non-woven fabric and modified polyurethane foam as carriers, respectively, were used to treat nitrogenous wastewater. Nitrogen removal performances of both reactors were investigated while the temperature decreased from 35℃ to 15℃. When the nitrogen loading rate (NLR) was 110 mg·(L·d), the average total nitrogen (TN) removal rate of the non-woven fabric reactor increased from 71.16% (35℃) to 76.19% (30℃) and then decreased to 61.11% (15℃). The average TN removal rate of the modified polyurethane foam reactor increased from 78.71% (35℃) to 81.75% (20℃) and then decreased to 75.68% (15℃). Compared with the non-woven fabric, the modified polyurethane foam provided a more stable nitrogen removal performance and could be used as the preferred carrier. High-throughput sequencing of the modified polyurethane foam biofilm indicated that was the dominant ANAMMOX genus, with relative abundances of 8% (35℃), 5% (25℃), and 16% (15℃). still grew and enriched at low temperatures. The decrease in temperature significantly affected the biofilm microbial community structure.

摘要

为了实现厌氧氨氧化(ANAMMOX)工艺的常温及低温运行,分别采用以无纺布和改性聚氨酯泡沫为载体的序批式生物膜反应器(SBBR)处理含氮废水。在温度从35℃降至15℃的过程中,对两个反应器的脱氮性能进行了研究。当氮负荷率(NLR)为110 mg·(L·d)时,无纺布反应器的平均总氮(TN)去除率从71.16%(35℃)提高到76.19%(30℃),然后降至61.11%(15℃)。改性聚氨酯泡沫反应器的平均TN去除率从78.71%(35℃)提高到81.75%(20℃),然后降至75.68%(15℃)。与无纺布相比,改性聚氨酯泡沫具有更稳定的脱氮性能,可作为首选载体。对改性聚氨酯泡沫生物膜进行高通量测序表明, 是优势ANAMMOX菌属,相对丰度分别为8%(35℃)、5%(25℃)和16%(15℃)。 在低温下仍能生长和富集。温度降低显著影响了生物膜微生物群落结构。

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